OSPE Aspergillosis

Question 1

case

Answer 1

-immunocompromised patient
- persistent cough, chest pain, and shortness of breath.
-Imaging reveals multiple nodules in the lungs.
-shows septate
hyphae with acute-angle branching.
-recent chemotherapy for leukemia.

Question 2

direct microscopy

Answer 2

colony: A wet preparation stained with lactophenol cotton blue demonstrates septate hyphae and conidiophores

Question 3

Specimen

Answer 3

Exudates, bronchial washings and bronchoalveolar lavage

Question 4

stain

Answer 4

wet mount
preparation in 10% potassium hydroxide

Question 5

Tissue sections

Answer 5

stain: periodic acid Schiff
dichotomous branching at an acute angle

Question 6

Detection of galactomannan

Answer 6

in serum or bronchoalveolar lavage fluid is a marker for the diagnosis of invasive aspergillosis

Question 7

Serology:

Answer 7

Precipitating antibodies to aspergillus antigens can be demonstrated by counter-current immunoelectrophoresis, immunodiffusion and ELISA.

Question 8

other

Answer 8

PCR and nucleic acid sequence-based

Question 9

Skin tests

Answer 9

Dual immediate (after 15 minutes) and Arthus type (after 4-6 hours) skin test reactions which develop after the intradermal injection of Aspergillus spp. antigens
- diagnosis of allergic broncho-pulmonary aspergillosis.

Question 10

culture

Answer 10

sabouraud agar medium

fumigatus:smoky green,velvety
flavus:yellow-green velvety
niger:black,cottony