L5: Independant Methods and Important People Flashcards
1
Q
List 5 methods of conducting culture-independent studies.
A
NGHA D C
1. nucleic acid extraction (16S rRNA)
2. Gel electrophoresis
3. Hybridization
4. Amplification
5. denatured gradient gel electrophoresis
6. Cloning amplifying gene
2
Q
A key aspect in genomics
A
creating a large database and comparing novel species to it
3
Q
Key Genomic Methods
A
Microarray
metagenomics
metatranscriptomics
4
Q
What does metagenomics allow
A
compare, analyse, study while focusing on gene of interest
5
Q
Louis Pasteur
A
- Pasteurisation
- disproved spontaneous generation
6
Q
Koch’s Postulates
A
- A diseased organism must have abundant pathogen
- must be able to culture pathogen in pure culture
- use it to infect healthy patients and it causes the same disease
- must be able to re-isolate pathogen
7
Q
Sergei
A
- Winogradsky
- Nitrogen Fixation
- Enrichment
