L26

Question 1

What was the point of molecular phylogency of archaea from soil (purpose, question, hypothesis)

Answer 1

purpose: investigate te diversity of soil Archaea

question: are methanogens the only Archaea present in soil

hypothesis: methanogens are not the only Archaea that live in soils

Question 2

What was the experimental approach

Answer 2

1. isolated clones from uncultured soil Archaea using methods similar to DeLong
2. constructed a phylogenetic tree to compare archaeal sequences found in soil to known Archaea

Question 3

What were the results of the Bintrim

Answer 3

SCA is soil clone archea
1. there were expected to be methanogens but here were none because not deep enough

2. SCA/Group 1 marine was initially classified as a branch of Crenarchaeota but were likely a separate phylum
   ==>the new phlum was Thaumarch, Nitrosph
3. shown on phylogenetic tree however it was not detected were two new groups
   ===>Group 2 marine on same branch as thermoplasma

===>Korarchatoa: 4th archeal phylum

Question 4

What ecological role(s) do Thaumarchaeota/ Nitrososphaerota play in the soil and marine environments

Answer 4

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