methods of studying cells - 2

Question 1

magnification formula

Answer 1

magnification= size of image/size of real object

Question 2

define resolution

Answer 2

how detailed the image is

Question 3

magnification definition

Answer 3

how much bigger the image is than the specimen

Question 4

2 types of electron microscopes

Answer 4

transmission and scanning

Question 5

2 types of microscopes

Answer 5

optical and electron

Question 6

maximum resolution of optical microscopes

Answer 6

0.2 micrometers

Question 7

maximum magnification of optical microscope

Answer 7

x1500

Question 8

maximum resolution of electron microscope

Answer 8

0.0002 micrometers

Question 9

maximum magnification of electron microscope

Answer 9

x1,500,000

Question 10

TEMs advantages

Answer 10

high resolution images, shows small images

Question 11

TEMs disadvantages

Answer 11

can only be used on thin specimens
non-living specimens

Question 12

SEMs advantages

Answer 12

3D
used on thick specimens

Question 13

SEMs disadvantages

Answer 13

gives lower resolution
only used on non-living specimens

Question 14

4 steps of preparing a microscope slide

Answer 14

1. pipet small drop of water in the centre of the slide
2. tweezers place thin section of specimen on top of water drop
3. add a drop of stain
4. add a cover slip

Question 15

3 main stages of cell fractionation

Answer 15

homogenisation
filtration
ultracentrifugation