Reagents Used In Protein Chemistry Flashcards
Cyanogen bromide
It is used for breaking peptide bonds specifically on the carboxyl side that cleaves at methionine residue generating smaller peptide fragment
Urea
It is used for denaturation, solubilization and purification
Beta mercaptoethanol
It is used for the reduction of disulphide bond in protein
Trypsin
It is used in the enzymatic digestion of protein which cleaves peptide bonds on the carboxyl side of amino acids lysine and arginine
Performic acid
It enhances the solubility and ionization of proteins,peptides and other biomolecules during chromatographic separation
Chymotrypsin
It is used in the enzymatic digestion of protein which target peptide bonds adjacent aromatic amino acids
Dabsyl chloride
It is used for labeling and analyzing proteins enabling their chromatographic separation, detection and identification
Fluorodinitrobenzene
It is used to selectively modify and label the amino group of the N terminus or the side chain of lysine residue
6N HCl
It is used for the hydrolysis of protein
Ninhydrin
It is used to detect and visualize amino acids and peptides. It forma colored compounds
Hydroxylamine
It is used to cleave asparagine and glycine peptide bonds respectively
