Module 10- Immunomodulation of Probiotics Flashcards
Metabolic Interactions that benefit gut epithelium
SCFAs, Conjugation linoleic acid, Vitamin B complex
Ways Probiotics Preserve Epithelial barrier function
Cell surface factors, Secreted proteins, Soluble peptides, Unmethylated CpG DNA, Probiotic factors and signalling pathways
Bifidobacterium Effectors that Trigger Immunomodulation
Unmethylated DNA, serpin, serine-threonine-rich proteins, Pili proteins
Effectors in both Bifido and Lactobacillus
EPS, Bacteriocins, SCFA, PG, CM
Effectors in Lactobacillus
Fimbriae, serine/threonine rich proteins, Lactoepin, SpaCBA, teichoic and lipoteichic acid
Cell Surface Factors Examples
Mucin expression, LTA from L. johnsonii inhibit LPS induced IL8 by HT29 epithelial cells
LTA form L. Johnsonnii
Inhibit LPS induced IL8 from H29 epithelial cells
Requires direct contact between Lactobacilli and epithelial cells
Cell Surface Factors
Cell Surface Factors
Not identified yet
Cell Surface Factors Broad Examples
Fimbriae, CPS, Glycoproteins
Probiotic traits from exported surface/secreted proteins
Adhesion to intestine, immunomodulation, Cross-talking with host
Strain dependent
Mechanisms of secreted or exported proteins
Types of Exported Proteins
Exported proteins and Moonlightling proteins
Sequence of exported proteins
Export signals + surface-retention domains
Exported proteins found…
In CM/CW or surface or secreted
Moonlighting proteins
Cytoplasmic proteins that lack export/secretion domains
Moonlighting proteins found
Associated with bacterial surface or released into environment
Moonlightling proteins function
Mostly using in intracellular processes but found on membrane surface to carry out other functions is possible
Exported proteins classified by
Domains in AA sequence