Module 14 - Safety Flashcards
Mainly non-pathogenic
Lactobacillus, Lactococcus, Leuconostoc, oral strep, Bifidobacterium, Saccharomyces
Some opportunistic
Lactobacillus, Strep, Enterococcus
Haemolytic activity in
Enterococci
Safety projects in Europe
LABIP (LAB industrial platform) + PROSAFE + FAIR
PROSAFE
Biosafety evaluation of LAB for human consumption
FAIR
Functional food science
Qualified Presumption of Safety (QPS)
Status via flowchart given by European Union Scientific Committee on Animal Nutrition
Three Approaches to Safety Studies
(1) Intrinsic properties of strain
(2) Pharmacokinetics of strain
(3) Interactions between strain and host
Pharmacokinetics Studies (4)
Survival, activity on intestine, dose-response relationships, faecal and mucosal recovery
Enzymatic properties detrimental to host
Excessive deconjugation of bile salts or degradation of mucus
Additional mechanistic studies if…
Bacterial survival differs at different levels of intestine tract + translocation or colonisation properties differ + metabolites produced are different
Used to determine colonization properties
Transit markers
Used to predict fate of bacteria
In vitro models
Predict fate of bacteri
Sensitivity to gastric acid and bile
Pharmacokinetics of Probiotics
Fecal of intestinal intubation or colonic biopsy techniqutes + Transit Markers + fate of bacteria