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HEMATOLOGY - Lab > OFT > Flashcards

OFT Flashcards

1
Q

In Sanford method, what percentage of NaCl will be dispensed into the tubes?

A

0.5% NaCl

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2
Q

After labeling and adding NaCl and distilled water, what is the next steps in Sanford method?

A

Add a drop of blood to each tube then mix well. Let the tubes to stand @ RT for 2 hours

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3
Q

A slight pink coloration of the supernatant indicates what? How about a clear-red solution?

A

Initial hemolysis

Complete hemolysis

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4
Q

To calculate the percent NaCl, what should be done?

A

Multiply the indicated tube no. w/ hemolysis to 0.02

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5
Q

What is the preffered sample for OFT? With what tube?

A

Defibrinized blood

Heparin tube (green top)

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6
Q

Is distilled hyper tonic, isotonic, or hypotonic?

A

Hypotonic

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7
Q

Sediments on the bottom of the tube indicates what?

A

Slight hemolysis. There are still intact RBCs

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8
Q

Percentage of NaCl stock solution needed for Dacie’s method

A

1% NaCl

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9
Q

Which method is more superior than the other?

A

Dacie’s method

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10
Q

What does Dacie’s method use to measure the results?

A

Spectrophotometer

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11
Q

How many tubes is used for Dacie’s method? Sanford method?

A

12

14

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12
Q

In Dacie’s method, for how long are the tubes allowed to stand? At what temperature?

A

30 minutes @ RT

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13
Q

After letting the tubes stand, what is the next step in Dacie’s method?

A

Centrifuge the tubes for 5 minutes @ 2000 rpm

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14
Q

When using a spectrophotometer, absorbance is measured at ______

A

540 nm

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15
Q

What is used to measure the absorbance of a sample in Dacie’s method?

A

Spectrophotometer

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16
Q

What sample is used in when measuring in Dacie’s method?

A

Supernatant

17
Q

normal percent hemolysis for 0.30% NaCl

A

97-100%

18
Q

normal percent hemolysis for 0.50% NaCl

A

0-5%

19
Q

normal percent hemolysis for 0.65% NaCl

A

0%

20
Q

normal percent hemolysis for 0.40% NaCl

A

50-90%

21
Q

normal percent hemolysis for 0.45% NaCl

A

0-45%

22
Q

Test used determining if hereditary spherocytosis and autoimmune hemolytic anemia is positive or negative

A

Direct antiglobulin test (DAT)

HEMATOLOGY - Lab (3 decks)

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