Hemostasis Flashcards

1
Q

Why are platelets hard to count?

A

They easily disintegrate
Small, colorless, refractile bodies
Difficult to distinguish from debris
unevenly distributed in the blood
tend to attach to foreign surface
tend to clump with each other

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2
Q

What factors are present in fresh plasma?

A

Factor I, II, V, VII, VIII, IX, X, XI, XII, XIII

(All factors)

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3
Q

What factors are present in adsorbed plasma?

A

Factor I, V, VIII, X, XII, XIII

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4
Q

Factors absent in adsorbed plasma

A

Factor IX, X, VII, III

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5
Q

Factors present in aged plasma

A

Factor I, II, VII, IX, X, XI, XII, XIII

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6
Q

What factors are absent in aged plasma?

A

Factors V and VIII

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7
Q

Factors present in normal serum

A

Factor II (20%), VII, IX, X, XI, XII

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8
Q

Factors absent in the normal serum

A

Factor I, V, VIII, XIII

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9
Q

Why do normal serum and aged serum have an absence in Fibrinogen group?

A

They are consumed during the clotting process

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10
Q

What differenciates the normal serum from the aged serum?

A

Aged serum has no Factor II

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11
Q

What is the best indirect method of quantifying platelets?

A

Olef’s method

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12
Q

What is the most accurate method of counting platelets?

A

Direct method

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13
Q

What does the bleeding time test for?

A

Measures the time required for the bleeding to stop

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14
Q

What is the method of testing the bleeding time using the pricking of the earlobe or the finger?

A

Duke method

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15
Q

What is the normal value of duke method?

A

2-4 minutes

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16
Q

What test uses aspirin to check for von Willeband deficiency?

A

Aspirin tolerance test

17
Q

What needle gauge is used for coagulation samples?

A

20-gauge

18
Q

What needle gauge is used when collecting more than 20 mL of blood?

A

19-gauge

19
Q

What is the blood to anticoagulant ratio of 3.8% trisodium citrate?

A

4:1

20
Q

What is the blood to anticoagulant ratio of 3.2% trisodium citrate?

A

9:1

21
Q

What is the coating used in syringes and evacuation tubes?

A

Silicon

22
Q

NV for whole blood clotting time:

A

7-15 minutes

23
Q

NV for capillary clotting time:

A

2-4 minutes

24
Q

What is the gold standard for the direct method of testing for primary hemostasis?

A

Becker-Cronkite method