Module 16- T1D Only Flashcards
Between 2001 and 2009 there was a … increase in T1D for people under 20
21%
Contribution of microbial species to disease
Metabolic network + Dynamic equilibrium with themselves and our cells + Innate and acquired defensive systems imbalanced
Kill Beta cells
Induced T and B cells
Proof that microbes play role in T1D
Provide antibiotics –> No disease
Leaky gut and T1D steps
Antigen gets through leaky gut –> activate APC in lamina propria –> cytokine release –> T and B cell activation –> kill beta cells
BB-DR rats
Biobreathing drug resistant predisposed to diabetes but do not get it
BB-DP rats
Biobreathing diabetes prone rats that get diabetes between day 17 and 110
Microbes in BB-DP rats
Bacteroides - Xanthomonas + Acinetobacter
BB-DR rats
Lactobacillus- Clostridium, Flexibacter, Porphyromonas
454-Pyrosequencing
Less diverse microbiota in rats over time and less richness
Criteria for selection of microbiota transplant of Lactobacillus
From 500 colonies –> Found in humans, probiotic properties reported (tolerance to bile and acid), no antagonistic effect to other Lactobacillus species
Onset of T1D
Days 69-110 –> gibe bacteria orally pre-weaning or post-weaing (immediate or after start of solid food) –> only difference in post weaning
L. Johnsonii changes in microbiota bacterial counts?
No differences
RT-PCR
Culture Independent method to see change in microbiota
RT-PCR results
More Lactobacillus in healthy and Enterococci in diabetic