genome sequencing Flashcards
1
Q
what is the genome?
A
all of the genetic material of an organism within a cell in the nucleus
2
Q
what is genome sequencing?
A
working out the DNA base sequence for all the DNA in a cell.
3
Q
how is genome sequencing carried out?
A
includes the sanger method
constantly being changed and improved
is now automated.
4
Q
why are prokaryotes more useful in genome sequencing?
A
do not have any introns so the genome can be used to directly sequence proteins derived from the genetic code/proteome of the organisms
useful for identifying potential antigens for a vaccine.
5
Q
complex organisms- eukaryotes
A
eukaryotes contain:
introns
regulatory genes e.g. promoters and terminators
so genome cannot be used directly to sequence proteome.
