Biochemical Food Tests Flashcards
What reagent is used for test for reducing sugars?
Benedict’s reagent
Does benedict’s test require heating?
Yes
What is the colour change in the reducing sugar benedict’s test?
Blue to red
What is the method of testing for non-reducing sugars?
Benedict’s normal test
Using the solutions that tested negative:
boil with acid and neutralise , acid = HCL, alkaline = sodium hydrogencarbonate
heat with benedicts solution
red precipitate
What 3 reagents are used in the test for non-reducing sugars?
Benedicts
HCl
NaHCO3 - sodium hydrogen carbonate
What is the regnant used to test for starch?
Iodine
Does the test for starch require heating?
No
What is the colour change for the test for starch?
Orange-brown to blue-black
What reagent is used to test for proteins?
Biuret reagent
Does the test for proteins need to be heated?
No
What is the colour change for proteins test?
Blue to lilac
What is the method for testing for lipids?
Add ethanol to test tube
Shake
Add water
A cloudy white emulsion will form
Does the test for lipids need to be heated?
No
What reagent is used in the test for lipids?
Ethanol
What is the colour change in the test for lipids?
Blue solution to emulsion formed