Micropara lab midterms

Question 1

Simple staining uses how many dyes?

Answer 1

1

Question 2

What dyes are used in positive simple staining

Answer 2

Basic dyes

Question 3

What dyes are used in negative simple staining

Answer 3

Acidic dyes

Question 4

Use two or more dyes that will distinguish bacteria from each other because their walls have different reactions on dyes

Answer 4

Differential staining

Question 5

Using staining, what else can you see in the structural components of bacteria? Name 3

Answer 5

Flagella, capsules, and granules

Question 6

For positive staining, what color should the microorganism be?

Answer 6

dark blue to violet with a bright background

Question 7

For negative staining what color should the microorganism be?

Answer 7

Colored with a colorless wall in a dark background

Question 8

What color is a gram positive bacteria?

Answer 8

Blue to violet

Question 9

What color is a gram negative bacteria?

Answer 9

pink to red

Question 10

What is the mordant used for gram staining

Answer 10

Gram Iodine

Question 11

What is the primary stain used for gram staining

Answer 11

Crystal violet

Question 12

What is the decolorizer used for gram staining?

Answer 12

Ethanol

Question 13

What is the secondary stain used for gram staining?

Answer 13

Safranin

Question 14

What are the different forms for characterization of colonies

Answer 14

Punctiform, circular, filamentous, rhizoidal, irregular

Question 15

What are the different margins for characterization of colonies

Answer 15

Entire, undulate, lobate, filamentous, rhizoid

Question 16

What are the different Elevations for characterization of colonies

Answer 16

Flat, raised, convex, pulvinate, umbonate

Question 17

What are the different Surfaces for characterization of colonies

Answer 17

Smooth, contoured, concentric, wrinkled

Question 18

What are the different consistencies for characterization of colonies

Answer 18

Butyrous, viscid, brittle

Question 19

What are the different transparencies for characterization of colonies

Answer 19

Opaque, Translucent

Question 20

This occurs when microorganisms enter a culture medium in which a pure culture of microorganism is growing

Answer 20

Contamination

Question 21

Isolation of bacteria into ______ is the best way to study and characterize bacterial cultures

Answer 21

Pure form

Question 22

How is isolation of bacteria into pure form accomplished?

Answer 22

By spreading sample containing microorganisms on a solid medium so that a single cell occupies a well-isolated portion of agar surface

Question 23

What are cultures consisting of a single species

Answer 23

Pure cultures

Question 24

What are three common techniques used in isolation of bacteria

Answer 24

Streak plating, spread plating, and pour plating

Question 25

In this plating, inoculum is spread over the surface of the medium by the inoculating loop

Answer 25

Streak plating

Question 26

In this plating, it uses an L-shaped rod to spread the inoculum over the entire plate

Answer 26

Spread plating

Question 27

In this plating, a medium is poured into the plate with an inoculum and allowed to solidify

Answer 27

Pour plate

Question 28

What plating is used to isolate and purify bacteria?

Answer 28

Streak plating

Question 29

What plating is used to isolate aerobic microorganisms?

Answer 29

Streak and spread plating

Question 30

What plating is used to isolate anaerobic microorganisms?

Answer 30

Pour plate

Question 31

For streak and spread plating, you should incubate the plates in what manner?

Answer 31

Inverted manner

Question 32

Important criterion used to establish the quality of food and dairy products

Answer 32

Bacterial counting/ Measuring size of a microbial population

Question 33

Measurement of the size population is done by

Answer 33

Counting the number of individual cell or by counting the number of viable cells that can form colonies on agar suface

Question 34

This is also known as counting chamber, is easy, inexpensive and relatively quick

Answer 34

Direct count

Question 35

Direct count gives information on?

Answer 35

The size and morphology of microorganisms

Question 36

Also known as the plating technique and is the most widely used because of the ease in counting the colonies and the manipulation of microscope is not required

Answer 36

Viable Count

Question 37

What dilution is used for spread plate?

Answer 37

0.1

Question 38

What dilution is used for pour plate?

Answer 38

1.0