biotech applications

Question 1

totipotency

Answer 1

ability to generate whole new plant from explant

Question 2

plants that are genetically identical to parent plant are called as

Answer 2

somaclones

Question 3

tissue culture prepared food plants such as

Answer 3

tomato banana apple

Question 4

scientist have succeeded in culturing meristems of plants like

Answer 4

banana sugarcane potato

Question 4

somatic hybridisation

Answer 4

isolated protoplast from two varieties of plant and fused to get hybrid varieties eg; pomato

Question 5

father of green revolution, world wide

Answer 5

norman e borlaug

Question 5

father of green revolution, India

Answer 5

ms Swaminathan

Question 6

genetic modification has made,

Answer 6

crops more tolerant to abiotic stress
pest resistance crops
reduce post harvest loss
increase efficiency of mineral usage by plants
enhance nutritional value of food

Question 7

beta carotene[ daffodils{ narcissur pseudonarcissus} ] is the precursor of

Answer 7

vitamin A[ night blindness/xerophtalmia]

Question 7

hirudin,

Answer 7

Anticoagulant
naturally taken from hirudinaria[annelida]

Question 8

Bacillus thuringenus

Answer 8

cry gene prepared
inactive insecticidal protein[ Bt toxin/ prototoxin]

Question 9

cry 1 Ab gene specific for

Answer 9

corn borer

Question 10

bt is produced protein that kill certain insects such as

Answer 10

lepidopterans [ tobacco budworm ,army worm], coleopterans [beetles],dipterans[flies, mosquitoes ]

Question 11

cry 2 Ab+ cry 1 Ac gene specific for

Answer 11

cotton ballworm

Question 12

inactive prototoxins are converted to an activated form of toxin due to

Answer 12

alkaline PH of the gut which solubilises the crystal and it create pores that cause cell swelling and lysis

Question 13

which nematode infects the roots of tobacco plant

Answer 13

meloidegyne incognita

Question 14

RNAi

Answer 14

takes place in all eukaryotes as a cellular defence . this method involve silencing of mRNA of the nematode due to its dsRNAS that binds to prevent translation

Question 14

how many recombinant therapeutic have been approved for humans

Answer 14

30 , in india 12

Question 15

earlier insulin was from

Answer 15

cattle and pig

Question 16

recombinant insulin manufactured by Eli Lilly on

Answer 16

1983

Question 17

functional ADA is introduced to lymphocyte using vector

Answer 17

retroviral vector

Question 17

first clinical gene therapy was given in year, to?

Answer 17

1990 , 4 year old girl [ADA{adenosine deaminase
} deficiency{SCID} ]

Question 17

for permanent cure ADA should be introduced during

Answer 17

embryo stage

Question 18

early diagnosis can be done by

Answer 18

PCR , ELISA, recombinant DNA technology

Question 19

PCR used to detect

Answer 19

HIV in AIDS patient, detect mutations in genes of cancer patients

Question 20

PROBE

Answer 20

ssRNA/ ssDNA tagged with radioactive molecule

Question 20

clone having mutated gane will not appear in

Answer 20

photographic film

Question 21

principle of ELISA

Answer 21

antibody-antigen interaction

Question 21

95% of transgenic animals are

Answer 21

mice

Question 22

transgenic model exist for many disease such as

Answer 22

cancer , cystic fibrosis, rheumatoid arthritis ,alzeimers

Question 23

alpha 1 antitrypsin is the protein used to treat

Answer 23

emphysema

Question 23

transgenic sheep

Answer 23

tracy

Question 23

first transgenic cow and gene has

Answer 23

1997,rosie cow, alpha lactalbumin

Question 24

who makes decisions regarding validity of GM research and safety of introducing GMO for public services

Answer 24

genetic engineering approval committee GEAC

Question 25

BIOPIRACY

Answer 25

use of bio resources by multinational companies and other organisations without proper authorisation from the countries and the people concerned without compensatory payment

Question 26

patent done in basmati rice, turmeric , neem

Answer 26

2 lack varieties of rice available and 27 are basmati rice

Question 26

year in which US took patent of basmati rice

Answer 26

1997

Question 27

the Indian parliament has recently has cleared the ——– of the Indian patent bill

Answer 27

second amendment