16 Flashcards
variolation
inoculation of dried pus from smallpox pustules onto the arm of a healthy individual
cardinal rule of workable vaccine
must contain antigen but not cause disease
first vaccine: when and who
edward jenner 1796
passived immunization
given antibodies
active immunization
given antigens
Immune serum globulin (ISG) aka gamma globulin
immunoglobulin extracted from pooled blood of at least 1,000 human donors-concentrates the antibodies and removes pathogens-Prevention of Hep A and measles-Replaces antibodies in immunodeficient pateints-injected intramuscularly-protection for 2-3 months
specific immune globulin (SIG)
immunoglobulin derived from more defined group of donors-obtain serum from patients who are convalescing and in a hyperimmune state-Used against pertussis, rabies, tetanus, chickenpox, and Hep B-preferred to ISG b/c higher titer counts
can antiseri and antitoxins from animal origin be used?
YesSera from horses is used against diptheria, botulism, and spider and snake bites-may cause serum sickness or anaphylaxis though
vaccination
exposing to antigenic, but not pathogenic materia-cause primary and anamnestic response for long term immunity
most vaccine stimulants contain:
1)killed whole cells or inactivated viruses2)live attenuated cells or viruses3)antigenic components of cells or virusesand/or4)genetically engineered microbes or microbial antigens
Killed or inactivated viruses
treatment with formalin, radiation, heat, or some other agent that does not destroy antigenicity
Vaccines of killed microbes require what
higher doses and more boosters because they do not multiply
which vaccines contain inactivated viruses
salk polio and influenza vaccine
Attenuation
process that substantially lessens the virulence of microbes-typically by modifying growth conditions or manipulating microbial genes -methods: long term cultivation, selection of cold mutants, passage of microbe through unnatural hosts and removal of virulence genes
Vaccines that have attenuated microbes
BCG for TB, measles, mumps, polio, and rubella
benefits of attenuated vaccines
1)microbes can multiply and cause infection but not disease2)long-lasting protection3)few doses and boosters
disadvantages of attenuated vaccines
1)require special storage2)transmitted to others3)mutate back to virulent strain
Acellular or subcellular vaccines
Used if exact antigenic determinants of BACTERIA that stimulate immunity are known.
subunit vaccines
Used if exact antigenic determinants of VIRUSES that stimulate immunity are known.
subunit/subcellular vaccines synthesis
1)taken from cultures of microbes2)produced by rDNA tech.3)synth chemically
Examples of component antigens
capsules of pneumococcus and meningococcus-protein surface antigen of anthrax and Hep B
Toxoid
special type of vaccine that consists of purified bacterial exotoxin that has been chemically denatured-provide protection against toxinoses like diptheria and tetanus
recombinant vaccines
made by isolating a gene for antigenicity from pathogen and splicing into a plasmid then recombining in a yeast