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MTAP 1 Myco > 1.6.1 Laboratory Diagnosis of Fungal diseases (Microscopic examination) > Flashcards

1.6.1 Laboratory Diagnosis of Fungal diseases (Microscopic examination) Flashcards

1
Q

Used for melanin pigment

a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

h

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2
Q

For tinea Versicolor
a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

f

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3
Q

What is the positive indicator for Acridine orange?

A

Green fluorescence (Fungi)

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4
Q

BEST fungal stain for tissues
a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

e

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5
Q

Hyphal elements

a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

a

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6
Q

Excellent mounting medium for most fung; most widely used stain

a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

c

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7
Q

Aman medium contains Lactic acid, phenol, and Cotton Blue

T or F

A

T

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8
Q

Stains Chitin; Bluish white/Apple green (+)

A

Calcofluor white

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9
Q

Demonstrates Encapsulated yeast

A

India ink (1 min)

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10
Q

Differentiation of Fungi tissue scrapings

a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

d

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11
Q

Rapid detection of fungi in skin, hair, nails

a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

b

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12
Q

Clearing agent that can remove debris within 10 mins

a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

b

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13
Q

How to increase rate of clearing for 10% KOH?

A

Heat

40% DMSO

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14
Q

In Hucker’s Modification (Gram stain) What is the primary stain?

a. Crystal Violet
b. Crystal Violet and Ammonium oxalate
c. Crystal Violet and H2O
d. Crystal Violet and Ammonium citrate

A

b

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15
Q

Mins for Gram stain?

A

3

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16
Q

Fungi are always Gram?

A

Pos

17
Q

Positive indicator for Periodic acid Schiff (PAS)

A

Magenta

18
Q

Candida, cervicovaginal Smear

a. Saline Mount
b. 10% KOH
c. Lactophenol cotton Blue (Aman Medium)
d. PAS
e. Grocott Methenamine Silver (GMS)
f. Acridine Orange
g. Pap’s
h. Masson Fontana

A

g

19
Q

GMS positive indicator?

A

Black

MTAP 1 Myco (34 decks)

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