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MTAP 1 Myco > 2.2.1 Lab diagnosis (Microscopy, Viral antigen detection) > Flashcards

2.2.1 Lab diagnosis (Microscopy, Viral antigen detection) Flashcards

1
Q

This type of microscope uses negative stain which provides a detention limit >10^6 to 10^7 mL; useful for nonculturable viruses

A

Electron Microscope

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2
Q

Light microscope can be used to see cytopathic effects of Viruses

T or F

A

T

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3
Q

Lipschultz Body, Henderson Patterson Bodies

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

A

d

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4
Q

Round, Swollen Refractile cells, occasionally Syncytia

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

A

b

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5
Q

Tzanck smear Detects Multinucleated Giant Cells in
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

A

b

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6
Q

Discrete foci of rounded, swollen, refractile cells, Slowly involves entire monolayer

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

A

c

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7
Q

MULTINUCLEATED GIANT CELL

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

A

b

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8
Q

Smudge cells

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

A

a

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9
Q

Rounding and aggregation of infected cells in GRAPE LIKE

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

A

a

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10
Q

Decoy cells in Urine Specimen

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

A

e

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11
Q

Owl’s Eye nuclear inclusion

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

A

a

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12
Q

Bizzare astrocytes, Oligodendrocyte nuclei enlarged foci of demyelination in white matte

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

A

d

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13
Q

Koilocytes

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

A

b

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14
Q

Enlarged group glass nuclei with chromatin precipitation in erythroid precursors

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

A

c

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15
Q

Negri Bodies

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

A

b

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16
Q

Characteristic refractile angular or tear shaped CPE; progress to involve entire

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

A

a

17
Q

Synctia formation

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

A

e

18
Q

Destructive degeneration with swollen, Vacuolated cells

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

A

d

19
Q

Flower Nuclei in T cells

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

A

c

20
Q

Syncytia in Hep-2

a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

A

b

21
Q

Characteristic refractile rounding cells
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

A

c

22
Q

Warthin Finkeldey cells

a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

A

d

23
Q

Councilman bodies
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

A

e

24
Q

CPE is usually absent or minimal

a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

A

a

25
Q

DFA and IFA uses fluorescence labeling, Which of the following is significant?

a. (-) No apple green Fluorescence
b. 1+ Fairly yet unequivocal apple green Fluorescence
c. 2+ apple green Fluorescence
d. 3+ Bright apple green Fluorescence
e. 4+ Brilliant apple green Fluorescence

A

c, d, e

26
Q

Enzyme linked Virus inducible System (ELVIS) is used for HSV

T or F

A

T

27
Q

Gold standard for Viral Diagnosis

a. Molecular methods
b. Microscopy
c. Cell culture
d. Serology

A

c

28
Q

Cell culture contains cell monolayer and cell culture medium

T or F

A

T

29
Q

What is the pH indicator for Cell culture medium?

A

Phenol red

MTAP 1 Myco (34 decks)

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