2.2.1 Lab diagnosis (Microscopy, Viral antigen detection)

Question 1

This type of microscope uses negative stain which provides a detention limit >10^6 to 10^7 mL; useful for nonculturable viruses

Answer 1

Electron Microscope

Question 2

Light microscope can be used to see cytopathic effects of Viruses

T or F

Answer 2

T

Question 3

Lipschultz Body, Henderson Patterson Bodies

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

Answer 3

d

Question 4

Round, Swollen Refractile cells, occasionally Syncytia

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

Answer 4

b

Question 5

Tzanck smear Detects Multinucleated Giant Cells in
a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

Answer 5

b

Question 6

Discrete foci of rounded, swollen, refractile cells, Slowly involves entire monolayer

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

Answer 6

c

Question 7

MULTINUCLEATED GIANT CELL

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

Answer 7

b

Question 8

Smudge cells

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

Answer 8

a

Question 9

Rounding and aggregation of infected cells in GRAPE LIKE

a. Adenovirus
b. Herpes Simplex Virus
c. Varicella Zoster Virus
d. Molluscum Contagiosum
e. Vaccinia, Variola

Answer 9

a

Question 10

Decoy cells in Urine Specimen

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

Answer 10

e

Question 11

Owl’s Eye nuclear inclusion

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

Answer 11

a

Question 12

Bizzare astrocytes, Oligodendrocyte nuclei enlarged foci of demyelination in white matte

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

Answer 12

d

Question 13

Koilocytes

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

Answer 13

b

Question 14

Enlarged group glass nuclei with chromatin precipitation in erythroid precursors

a. Cytomegalovirus
b. HPV
c. Parvovirus B19
d. JC virus
e. BK virus

Answer 14

c

Question 15

Negri Bodies

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

Answer 15

b

Question 16

Characteristic refractile angular or tear shaped CPE; progress to involve entire

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

Answer 16

a

Question 17

Synctia formation

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

Answer 17

e

Question 18

Destructive degeneration with swollen, Vacuolated cells

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

Answer 18

d

Question 19

Flower Nuclei in T cells

a. Entero virus
b. Rabies
c. HTLV-1
d. Influenza
e. Mumps

Answer 19

c

Question 20

Syncytia in Hep-2

a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

Answer 20

b

Question 21

Characteristic refractile rounding cells
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

Answer 21

c

Question 22

Warthin Finkeldey cells

a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

Answer 22

d

Question 23

Councilman bodies
a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

Answer 23

e

Question 24

CPE is usually absent or minimal

a. Parainfluenza
b. Respiratory Syncytial virus
c. Rhinovirus
d. Measles
e. Yellow fever

Answer 24

a

Question 25

DFA and IFA uses fluorescence labeling, Which of the following is significant? a. (-) No apple green Fluorescence b. 1+ Fairly yet unequivocal apple green Fluorescence c. 2+ apple green Fluorescence d. 3+ Bright apple green Fluorescence e. 4+ Brilliant apple green Fluorescence

Answer 25

c, d, e

Question 26

Enzyme linked Virus inducible System (ELVIS) is used for HSV T or F

Answer 26

T

Question 27

Gold standard for Viral Diagnosis a. Molecular methods b. Microscopy c. Cell culture d. Serology

Answer 27

c

Question 28

Cell culture contains cell monolayer and cell culture medium T or F

Answer 28

T

Question 29

What is the pH indicator for Cell culture medium?

Answer 29

Phenol red