Additional Scientists Flashcards
Who’s work did Hotchkiss continue?
Avery-Macleod-McCaferty
What did Edwin Chargaff discover? (2 things)
Bases are not found in equal proportions
Base composition varied between species
What did Rollin Hotchkiss do?
Purified transforming material so that it contained only 0.02% protein
The transforming ability was retained
What did Hotchkiss work show?
That genetic traits other than r-strain and s-strain could be transferred by DNA
What question did Hersey-chase experiment answer? What was the answer?
Do the genes reside in DNA or protein?
DNA
Describe the first Hershey-chase experiment
The protein coat was labeled specifically with 35-sulfur
Attachment of phage to host cell
Removal of page coats by blending
Resulting in Cell containing little 35S-labeled protein plus unlabeled DNA
Describe the second Hershey-chase experiment
DNA is labeled specifically with 32 phosphorous protein
Attachment of phage to host cell
Removal of phage coats by blending
Resulted in cell containing little 32P-labeled protein
What do phases do in the host cell?
Enter the host cell and direct the synthesis of new phage particles
What are phases composed of?
Only DNA and protein
The _______ was used in the Hershey-chase experiments
T2 bacteriophage
What part of the phage was labeled with 32P?
Phage DNA
What part of the phage was labeled with 35-S
Phage protein
There is a lot of _______ in DNA and no ______
Phosphorous
Sulfur
There is a lot of _______ in phage protein and no _______
Sulfur
Phosphorous
What amino acids contain sulfur?
Methionine and cysteine
