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Question 1

What is the significance of the C-terminus in Srcs?

Answer 1

If phosphorylated, Src is inactive.

If dephosphorylated, Src becomes active, ready to phosphorylate other proteins.

Question 2

What are the functions of the 4 different SH domains?

Answer 2

SH1: The catalytic domain where Src adds phosphate groups to proteins (this is the actual kinase part).

SH2: This domain binds to phosphorylated tyrosine residues on other proteins—this is how Src finds its targets.

SH3: Facilitates protein-protein interactions with sequences that are rich in proline (a specific amino acid).

SH4: A unique domain whose function isn’t fully understood, but it’s involved in linking Src to the plasma membrane.

Question 3

Give me examples of cytokine receptors that rely on non-receptor tyrosine kinases

Answer 3

IL-1, IL-2, IL-6

Question 4

Give me examples of hormone receptors that rely on non-receptor tyrosine kinases

Answer 4

Prolactin, Leptin, Growth Hormone

Question 5

Give me two examples of non-receptor tyrosine kinase

Answer 5

Src

JAK

Question 6

What are examples of adaptor molecules that contain SH2?

Answer 6

Src

JAK

Abl

Tec

Question 7

What is an example of an adaptor molecule that doesn’t contain SH2?

Answer 7

Ras

Question 8

What is the structure of the insulin receptor?

Answer 8

Alpha subunit:

Extracellular and where insulin binds

Beta subunit:

Intracellular and contains tyrosine kinase domains

Question 9

What type of bonds connect the alpha and beta subunits in the Insulin receptors?

Answer 9

Di sulfide bonds