2/26 German B Cells Antibodies n Diversity

Question 1

Phase 1

Answer 1

Repertoire Assembly

Question 2

Phase 2

Answer 2

Negative Selection

Question 3

Phase 3

Answer 3

Positive Selection

Question 4

Phase 4

Answer 4

Searching for Infection

Question 5

Phase 5

Answer 5

Finding Infection

Question 6

Phase 6

Answer 6

Attacking INfection

Question 7

Effector FUnctions of antibodies

Answer 7

Receptors
Neutralization
Opsonization
SIgnaling

Question 8

What are the 3 sources of antigen diversity

Answer 8

1) Genetc Recombination
2) Junctonal Diversity
3) Somatic Hypermutation

Question 9

What determines antbody class

Answer 9

Isotype

Question 10

5 Isotypes are

Answer 10

MD

GEA

Question 11

What region binds epitope?

Answer 11

Variable

Question 12

What region is the variable region? Fab or Fc

Answer 12

Fab

Question 13

LIght CHain is a proportionally _________ variable region

Answer 13

Large

Question 14

Two isotypes of LC

Answer 14

Kappa

Lambda

Question 15

What protein is cleaved in order to make Fab and Fc complexes

Answer 15

IgG

Question 16

The five antbody classes are dependent on ___?

Answer 16

The HC

Question 17

What are the multimeric complexes

Answer 17

Pentameric IgM

Dimeric IgA

Question 18

Antingen (s) best at neutralization

Answer 18

IgG

IgA

Question 19

Antingen (s) best at Opsonization

Answer 19

IgG

Question 20

Antingen (s) best at sensitization of mast cells

Answer 20

IgE

Question 21

Antingen (s) best at Sensitization of basophils

Answer 21

IgD

Question 22

Antingen (s) best at Activation of complement

Answer 22

IgM

IgG

Question 23

Antingen (s) best at Transport across epithelium

Answer 23

IgA

Question 24

Antingen (s) best at Transport across placenta

Answer 24

IgG

Question 25

Antingen (s) best at diffusion into extravascular sites

Answer 25

IgG

Question 26

HV means

Answer 26

Hypervariable

Question 27

CDR means

Answer 27

COmplementary - determining REgion

Question 28

4 types of epitope terminals

Answer 28

1) Terminal Polysaccharide 2) Polysaccharide chain 3) Globular Protein Surface 4) Globular Protein Pocket

Question 29

4 types of epitope shapes

Answer 29

Linear Discontinuous Multivalent antigen with multiple epitopes Multivalent agent with repeateing epitope

Question 30

4 structure changes in flexibility that IgG has

Answer 30

1) Wave 2) Rotate 3) Wag 4) Bend

Question 31

Whats the order from right to left of location of gene segments on DNA

Answer 31

COnstant Joining Diversity Variable

Question 32

How many Variable in LC?

Answer 32

Kappa: 34 Lambda: 29

Question 33

How many variable in HC

Answer 33

38

Question 34

How many diversity in HC

Answer 34

23

Question 35

How many diversity in LC

Answer 35

0

Question 36

How many joininc in LC

Answer 36

Kappa: 5 Lambda: 4

Question 37

How many COnstant in LC

Answer 37

Kappa: 1 Laambda: 4

Question 38

How many constant in HC?

Answer 38

9

Question 39

In how many human chromosomal locations for genetic recombination?

Answer 39

3

Question 40

How are segments paired in Somatic Recombination?

Answer 40

Randomly but process follows a set sequence

Question 41

WHat binds RSS during Junctional diversity?

Answer 41

RAG 1 and 2

Question 42

How many base pairs are RSS?

Answer 42

7 bp | 9bp

Question 43

What is the process of Junctional DIversity? Describe the steps

Answer 43

1) RAG cleaves heptamer RSS 2) DNA haipins are left 3) RAG opens hairpins, which generates palindromic p-nucleotides 4) N-Nucleotides are added by TdT 5) Strands are paired and unpaired nucleotides removed by EXONUCLEASE 6) Gaps filled by DNA synthesis matching comple,ment nucleotide

Question 44

Whats the order of terminal path of isotype

Answer 44

MD GEA

Question 45

Is isotype switching permanent?

Answer 45

YES!!

Question 46

What are the 2 isotypes first expressed?

Answer 46

MD

Question 47

What receptors bind conserved regions?

Answer 47

Fc

Question 48

What isotypes can B cells express?

Answer 48

All ab isotypes

Question 49

What are the only two changes to the B Cell's genome that are reversible

Answer 49

1) Transcription activated with expression of IgM and IgD | 2) Changes from membrane Ig to secreted antibody

Question 50

Polyclonal def

Answer 50

Multiple antibodies targeted against same antigen

Question 51

Monoclonal def

Answer 51

Single antibody targeted against single antigen

Question 52

DIagnostic antibody functions

Answer 52

1) Pathogen identification 2) Protein quantification 3) Cellular ID

Question 53

Therapeutic antigen funtion

Answer 53

1) Targeted Killing 2) Chemical delivery 3) Immunomodulatory