2 - EXAMINATION OF FECAL SPECIMENS

Question 1

Contains bacteria, cellulose, and other undigested foodstuffs, GI secretions, bile pigments, cells and electrolytes, and water

Answer 1

Feces

Question 2

Around ?g of stool is passed per day

Answer 2

100-200g

Question 3

• Most common method of diagnosis in parasites (protozoans and metazoans)
• Best collected in clean, wide-mouthed containers made of waxed cardboard or plastic with tight-fitting lid to ensure retention of moisture and prevent spillage

Answer 3

Examination of Fecal Specimens

Question 4

Parasite Stages Detected

Answer 4

Cyst
Trophozoites
Oocyts
Eggs
Larvae
Adult worm

Question 5

Clearance period following ingestion of certain drugs and compounds:

Oily materials, non-absorbable antidiarrheal preparations, barium, bismuth, or antimalarial agents

Answer 5

7 days

Question 6

Clearance period following ingestion of certain drugs and compounds:

Antimicrobial agents

Answer 6

2-3 weeks

Question 7

Clearance period following ingestion of certain drugs and compounds:

Gallbladder dyes

Answer 7

3 weeks

Question 8

amount of stool

Formed

Answer 8

2-5 grams

Question 9

amount of stool

Watery

Answer 9

5-6 tablespoons

Question 10

Liquid specimen should be examined within ? after passage

Answer 10

30 minutes

Question 11

Soft specimen should be examined within ? after passage

Answer 11

1 hour

Question 12

Formed specimen should be examined within ? with refrigeration after passage

Answer 12

24 hours

Question 13

Order of processing:

Answer 13

Bloody/Mucoid
Watery
Formed

Question 14

Color

Answer 14

Brown
Dark brown
Light brown

Question 15

Consistency

Answer 15

Formed
Soft
Loose
Watery

Question 16

Check for the following:

Answer 16

• mucus
• blood
• larval or adult worms
• proglottids

Question 17

Most easily performed parasitologic test

Answer 17

Direct Wet Mount / Direct Fecal Smear

Question 18

DFS is most useful when fresh specimens, especially liquid stools or duodenal aspirates, are examined for

Answer 18

motile trophozoites or helminth larvae

Question 19

DFS is for screening or diagnosis?

Answer 19

Screening test only

Question 20

Small amount of stool is mixed with a drop of ? and ? and covered with a coverslip

Answer 20

0.85% saline, Lugol’s iodine

Question 21

may be used in the event that a protozoan trophozoite is found

Answer 21

Buffered Methylene Blue

Question 22

Wait for ? before examining to allow the stain to penetrate the trophozoite for about ?

Answer 22

5-10 minutes, 30 minutes

Question 23

If parasite ova or larvae are seen, report as:

Answer 23

Positive for species and stage of the parasite
Ex: Positive for Ascaris lumbricoides egg

Question 24

If parasite ova or larvae are NOT seen, report as:

Answer 24

No Intestinal Parasite Seen (NIPS)

Question 25

saline smear findings

Answer 25

Huge no. of:
Eggs
Protozoan trophozoite motility (Amoebas, flagellates)

Question 26

iodine smear findings

Answer 26

Huge no. of:
Cyst morphological details