3 - KATO THICK SMEAR

Question 1

Simple technique for mass stool examinations
Good for detecting eggs with thick egg shells

Answer 1

Kato Thick Smear

Question 2

amount of stool needed for kato thick

Answer 2

50-60mg of stool

Question 3

materials of kato thick

Answer 3

Cellophane
Glycerine
Malachite green solution

Question 4

acts as a coverslip

Answer 4

Cellophane

Question 5

clearing agent

Answer 5

Glycerine

Question 6

staining agent

Answer 6

Malachite green solution

Question 7

Advantages

Answer 7

Time saving, simple and economical
Adapted to mass examination
Satisfactory for all common types of helminth eggs

Question 8

Disadvantages

Answer 8

Unsuitable for diarrheic stools
In some stools (especially infants), fermentation gas is troublesome
Cannot be used to diagnose protozoan cysts and trophozoites

Question 9

Check the color and consistency of stool
Collect 50-60mg of stool (size of two mongo beans)
Place the soaked cellophane on the stool
Using the rubber stopper, press the stool sample evenly
Cover the whole cellophane with stool
Label the specimen (name, date and time)
Allow the specimen to settle for an hour or two before viewing under the microscope (no need for cover slip)

Answer 9

Procedure

Question 10

If parasite egg or larvae are seen, report as:

Answer 10

Positive for species and stage of the parasite
Ex: positive for Ascaris lumbricoides egg

Question 11

If parasite egg or larvae are NOT seen, report as:

Answer 11

No Intestinal Parasite Seen (NIPS)