[23] Cre-Lox Recombination Flashcards
(21 cards)
What is Cre-Lox recombination?
Site-specific recombinase technology for DNA manipulation.
Is Cre-Lox specific to eukaryotic systems?
No, both eukaryotic and prokaryotic systems.
Where is the Cre enzyme derived from?
Bacteriophage P1
What is the role of Lox sequences?
Target sequences for Cre recombinase.
What is the original Lox site called?
LoxP
What has Cre-Lox been useful for in neuroscience?
Studying complex cell types and neural circuits.
How has the NIH contributed to Cre-Lox research?
Created Cre driver mouse lines for global use.
What problem can Cre-Lox solve in gene replacement?
Removal of selectable markers affecting gene expression.
In what organisms is Cre-Lox widely used?
- Plants
- Mouse cell lines
- Yeast
What types of genomic changes can Cre-Lox implement?
- Deletions
- Insertions
- Translocations
- Inversions
What enzyme carries out Cre-Lox recombination?
Cre recombinase
What are loxP sequences?
Specific binding sites for Cre surrounding a directional core.
What happens when Cre and loxP interact?
Recombination event between loxP sites.
How is the orientation of loxP sites important?
- Inversion (inverted)
- Deletion (direct repeat)
How can Cre recombinase be controlled?
- Cell-specific promoters
- External stimuli
What is the structure of Cre protein?
- Larger C-terminal domain
- Smaller N-terminal domain
What is loxP?
34 bp site on bacteriophage P1 with specific sequences.
What is the result if loxP sites are in opposite orientation?
The floxed sequence is inverted.
NIH - Initials
National Institutes of Health
bp - Initials
Base Pairs
Cre - Initials
Causes Recombination
