DNA Technology

Question 0

What are single strands?

Answer 0

Unpaired nucleotides produced by the action of restriction enzymes

Question 1

In order for a foreign gene to be inserted into a plasmid, both must _________.

Answer 1

Be cut by the same restriction enzyme

Question 2

Why do restriction fragments cut from DNA by restriction enzymes separate during gel electrophoresis?

Answer 2

Because shorter fragments travel faster

Question 3

What is the final step to finish insertion after a gene has been inserted into a plasmid?

Answer 3

DNA Ligase

Question 4

What would a cDNA version of a gene include?

Answer 4

Codons for a mature mRNA

Question 5

What do bacteria use restriction enzymes to do?

Answer 5

Destroy foreign DNA

Question 6

DNA synthesized using a known mRNA structure is called____.

Answer 6

cDNA

Question 7

Genetic engineering manipulates gene products at the level of the_____.

Answer 7

DNA

Question 8

Why have RFLPs been tremendously useful for gel electrophoresis studies?

Answer 8

They are not restricted to specific genes, and are scattered throughout the genome

Question 9

What is a nuclear probe used to do?

Answer 9

Identify genes that have been inserted into bacterial plasmids or separated by electrophoresis

Question 10

What does gene therapy involve?

Answer 10

Adding a functioning version of a defective gene to the cells of an individual