24 Stem Cells Flashcards
(21 cards)
blastocyst undergoes
gastrulation
gastrulatioin
form three germ layers
ectoderm
outer skin
mesoderm
inner skiin
endoderm
digestive tract lining
embryonic stem cells (ESCs)
can be extracted from inner cell mass of blastocyst and grown
pluripotent
be able to specialize to different cells
self renewal
create more stem cells
cellular potency
cell’s potential for differentiation
sequence for potency
totipotent
pluripotent
multipotent
unipotent
totipotent
fertilized egg
pluripotent
blastocyst
multipotent
ectoderm, mesoderm, endoderm
unipotent
differentiated cells
pluripotent differential regulation
transcription factors, cofactors, chromatin regulators
What happens when Oct4 is on
loose chromatin
what happens when Oct 4 is off
condensed chromatin, differentiated cell
iPSCs
reprogrammed somatic cells to pluripotent state
stem cell niche
stem cells are in protected areas of an organ
lineage tracing
tracing cells to determine descendants of stem cells
organoids
researchers cna use stem cells to create complex organoids in vitro