culturing bacteria

Question 1

def of differential media

Answer 1

selective for group bacteria and contains indicator

Question 2

differential media (4)

Answer 2

MAC, TSI, Hektoen-Enteric agar, mannitol salt agar.

Question 3

macconkey

Answer 3

lactose fermenters will cause colonies to be pink. non-lactose will be clear.

Question 4

hektoen-enteric, what bacteria? picks up on what dz, what color?

Answer 4

G - enteric pathogens. good for salmonella. sugar fermenters turn salmon colored. non-sugar turn blue/green

Question 5

triple sugar iron

Answer 5

yellow indicates fermentation

red indicated no fermentation

Question 6

mannitol salt agar

Answer 6

selects for Staphylococci. isolate staph aureus
yellow- organism ferments mannitol
red- no staph

Question 7

2 selective medias

Answer 7

selenite broth and tetrathionate broth

Question 8

selective media contain what to allow bacteria to grow better

Answer 8

nutrients

Question 9

basic nutrient media with extra nutrients (blood, serum, egg)

Answer 9

enriched media

Question 10

what select bacteria for phenylethel alcohol agar and macconkey

Answer 10

PEA- gram +

MAC- gram - rods

Question 11

partial, narrow band of green/slimy around colony

Answer 11

alpha

Question 12

complete hemolysis- produce clear zone

Answer 12

beta

Question 13

no change in media surrounding colony

Answer 13

gamma

Question 14

double zone hemolysis.

Answer 14

delta

Question 15

which two differentiate strept. species

Answer 15

alpha and beta

Question 16

3 parts to the initial plating technique

Answer 16

protocol, contamination, application

Question 17

what is more permeable to most common abx

Answer 17

gram +

Question 18

3 types of zones of inhibition

Answer 18

sensitive- clear zone
intermediate- small clear zone
resistant- nothing. abx doesnt affect.

Question 19

lowest concentration of the abx that inhibits growth

Answer 19

minimum inhibitory concentration

Question 20

several types of culture media

Answer 20

agar plates, agar tubes, slant tubes, broth tubes.

Question 21

types of media (2)

Answer 21

enriched media, basic nutrient

Question 22

2 types of basic nutrient media

Answer 22

nutrient agar

trypticase soy agar

Question 23

2 types of enriched media

Answer 23

chocolate

blood agar plate

Question 24

2 solid selective media

Answer 24

PEA and MAC