2.6 Flashcards
(7 cards)
Three domains of life?
Archaea, bacteria, some eukaryotes
Conditions needed for micro organisms?
•Sterility
•Control of temperature
•Oxygen levels
• pH
Why are sterile conditions needed?
•reduce competition
•reduce spoilage
A growth media will
Provide the micro organisms with raw materials for biosynthesis as well as an energy source
Phases of growth media
•Lag phase - enzymes are induced to metabolise substrates
• log phase- most rapid growth of microorganisms due to plentiful nutrients
•Stationary phase - nutrients become depleted and production of toxic metabolites
•death - toxic accumulation of metabolites
Viable cell counts
Counting ONLY living micro organisms
(Show a death phase where numbers are decreasing)
Total cell counts
Viable and dead cells