2.7 Flashcards
(7 cards)
1
Q
Two ways in which wild strains are improved
A
Mutagenesis and recombinant DNA tech
2
Q
Mutagenesis
A
Exposing micro organisms to forms of radiation or mutagenic chemicals resulting in mutations
3
Q
Recombinant DNA tech invokes the use of:
A
•Recombinant plasmids
• Artificial chromosomes
4
Q
Vector
A
DNA molecule used to carry foreign info into another cell
5
Q
Restriction endonuclease
A
Cut open plasmids and leave sticky ends
6
Q
What is ligase used for
A
Seal the gene into the plasmid
7
Q
Four components that a plasmid or artificial chromosome must contain
A
• restriction site
• regulatory sequences
• origin replication
•selectable markers