Week 9 notes Flashcards
What are some ways to receive passive immunity
maternal antibodies passed to offspring via colostrum
or donor antibodies via transfusion/injection
define Adaptive immune system (humoral and Cell mediatied)
humoral - antibodies interact with antigents to prevent them from causing disease
Cell mediated - Direct destruction of antigens by cells
phagocytosis
lysis
What are some ways to receive active immunity
Exposure to disease
immunization
how does a serology test work
Antigen detection using antibodies
or antibody detection using antigens
What will positive serologic results produce
Color change
Light
A radiolabeled substance
What does ELISA stand for and how does it work
Enzyme linked immunosorbent assay
contains either an antibody or antigen, and an enzyme
antibodies and antigens bind together and react with the enzyme to produce a color change
What does CELISA stand for and how does it work
Competitive enzyme linked immunosorbent assay
contains BOTH antibodies and antigens
sample must compete with the antigen int eh test for binding sites to produce a color change
How does the latex agglutination test work
latex particles are coated with antigen
binding of matching antibodies and antigen case agglutination
What does RIM stand for and how does it work
Rapid immunomigration
Antibodies in the test kit are attached to colloidal gold
bound antibodies/antigen groups migrate to the reading area
Why would we perform a Coombs Test
Testing for IMHA- immune mediated hemolytic anemia (attacking its own RBC’s)
Allergy testing and Tuberculin testing are examples of what kind of tests
Intradermal testing
A positive tuberculin test would mean an exposure to what
M. tuberculosis
M. bovis
M. avium
A disease titer is being performed. What does a titer of 1:2 mean? What does a titer of 1:100 meant? Which one is “good” and which one is “bad”?
disease titer of 1:2 is “good” because it shows a high ratio of antibodies to the disease.
disease titer of 1:100 is “bad” because it shows a low ratio of antibodies to the disease
A vaccine titer is being performed. What does a titer of 1:2 mean? What does a titer of 1:100 mean? which one is “good” and which is “bad”?
a vaccine titer of 1:2 is “bad” because there is more disease when compared to the vaccine antibody
A vaccine titer of 1:100 is “good” because the disease is diluted and still showing the vaccine antibody
T/F “while a single positive serologic test for antibodies might indicate that an animal has been exposed to an infectious agent, this is never enough to make a definitive diagnosis.”
False
example: a positive heartworm test, a doctor will diagnose right then and there.
