27 Bacteria and Archaea Flashcards

(221 cards)

1
Q

What is a genus of microorganism that can tolerate wide salt ranges?

A

Halobacterium, which is actually a archaea

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is Halobacterium?

A

An archaea genus that can tolerate high levels of salt.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the basic premise of the the Gram stain?

A

It dies peptidoglycan as purple so that bacteria that have it on their plasma membrane can be distinguished from those who do not have it on their plasma membrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

How can bacteria be classed based on how they appear under a Gram stain?

A

‘Gram-positive bacteria’ and ‘Gram-negative bacteria’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the structure of gram-postive bacteria?

A

The differences lie in the plasma membrane. In Gram positive bacteria the plasma membrane is covered with an external layer of peptidoglycan, which acts a the cell wall.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is peptidoglycan?

A

A polymer composed of sugars cross-linked with polypeptides

It is the primary component of bacterial cell walls.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the structure of a gram-negative bacteria?

A

They have a phospholipid membrane bilayer that acts as the plasma membrane.

External to this is a comparatively thin layer of peptidoglycan.

External to this is an outer membrane, which consists of a phospholipid membrane bilayer.

Note that the peptidoglycan layer and the external phospholipid membrane bilayer are collectively called the ‘cell wall.’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Generally speaking, are Gram-negative or Gram-positive bacteria more resistant to antibiotics? Why

A

Generally Gram-negative bacteria as many antibiotics work by inhibiting the peptidoglycan cross linking (this allows them to not affect human cells, which lack peptidoglycan)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the cell wall of a bacterium covered with?

A

Either a ‘slime layer’ or a ‘capsule’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is a ’slime layer’?

A

An outer coating of a bacterium that is not dense or defined like a capsule

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is a ‘capsule’?

A

An outer coating of a bacterium that is very dense and defined, unlike a ’slime layer’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What can the slime coats/capsules of bacteria be covered with?

A

Fimbriae or pili

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is a pilus?

A

The singular form of pili

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are fimbriae?

A

Hairlike appendages that are shorter and more numerous than ‘pili’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are ‘pili’?

A

Appendages that pull to cells together prior to horizontal gene transfer between bacteria.

They are longer but less numerous than fimbriae.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is a taxis?

A

A directed movement toward or away from a stimulus.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What is a directed movement towards or away from a stimulus called?

A

A taxis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is a positive taxis?

A

When the bacterium etc. moves towards a stimulus i.e. positive phototaxis towards light

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What is the structure of a prokaryotic flagellum?

A

Embedded in the inner plasma membrane is a ‘motor’ which appears as a set of discs

In the centre of these disks is a ‘rod’ which extends outside the cell wall.

Just outside the cell wall the rod bends to form the ‘hook’. The ‘filament’ is the long trailing tail that extends form the hook

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What is exaption?

A

When evolution repurposes features for other uses.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Where is the DNA of a bacterium located?

A

In a ’nucleoid’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What is a ’nucleoid’?

A

A region in a bacterium’s cytosol which holds the chromosome.

Note that it is not membrane bound and is thus loosely defined.

It appears lighter than surrounding areas in an electron microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What is a common way that bacteria tolerate changing environmental conditions i.e. periodic drought?

A

When they lack certain nutrients or during harsh conditions they can become dormant ‘endospores’

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

What are ‘endospores’?

A

A dormant form that many bacteria can assume when they lack certain nutrients or under harsh conditions.

The original cell produces a copy of its chromosome and surrounds it with a tough multilayered structure, forming the endospore.

Water is removed from the endospore, and its metabolism halts. The original cell then lyses, releasing the endospore.

When dehydrated they become active bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Does meiosis occur in prokaryotes?
Nope
26
What are What is the ploidy of prokaryotes?
They are all haploid
27
What is 'genetic recombination’?
The combination of DNA from two sources
28
How does ‘genetic recombination’ occur in eukaryotes?
With fertilisation and in meiosis (crossing over)
29
What is the combination of DNA form two sources called?
Genetic recombination
30
How does genetic recombination occur in prokaryotes?
Since they do not perform meiosis or fertilisation it only occurs in ‘horizontal gene transfer'
31
What are the basic methods of horizontal gene transfer?
Transformation, transduction, conjugation and plasmids
32
What is ’transformation’ as a method of horizontal gene transfer?
The genotype and possibly phenotype of a prokaryotic cell are altered by the uptake of foreign DNA from its surroundings. For example transformation occurs when a nonpathogenic cell takes up a piece of DNA carrying the allele for pathogenicity and replaces its own allele with the foreign allele, an exchange of homologous DNA segments. The cell is now a recombinant.
33
What is ’transduction’ as a method of horizontal gene transfer?
Phages (bacteriophages = viruses that infect bacteria) carry prokaryotic genes from one host cell to another. If some of this DNA is then incorporated into the recipient cell’s chromosome by DNA recombination, a recombinant cell is formed.
34
What is ’conjugation’ as a method of horizontal gene transfer?
DNA is transferred between two prokaryotic cells (usually of the same species) that are temporarily joined. In bacteria, the DNA transfer is always one-way: One cell donates the DNA, and the other receives it. The tow bacteria are held together by a pilus
35
What are ’plasmids’ as a method of horizontal gene transfer?
Plasmids are small sections of DNA. They can be transferred between bacteria in ‘horizontal gene transfer'
36
In what form of horizontal gene transfer are pili (singular: plies) involved?
Conjugation
37
How are certain forms of horizontal gene transfer regulated?
With the ‘F factor'
38
What are the forms of F factor?
It is just F factor if it exists in the bacterial chromosome. If it is in its own plasmid it is the ‘F plasmid'
39
What forms of horizontal gene transfer does the F factor regulate?
Conjugation and Plasmid
40
How does the F factor regulate conjugation?
The F factor consist of genes needed for the formation of a pilus etc. Cells that have the F factor are called HFR (high frequency recombinant) During conjugation they act as donors and transfer genetic information to the other bacterium. Note that the HFR bacterium can transfer the F factor to the host F- bacterium, causing it to become F+ (HFR)
41
What does the ‘F’ in F factor stand for?
Fertility
42
What is a ‘F+’cell?
One with the F factor
43
What is a ‘F-‘ cell?
One without the F factor
44
What does ‘HFR’ refer to?
High frequency recombination. It refers to a bacteria which has the F factor in its chromosome and thus is F+
45
How does can the F plasmid be transferred to another bacterium?
A cell carrying an F plasmid (an F+ cell) forms a mating bridge with an F– cell. One strand of the plasmid’s DNA breaks at one point. Using the unbroken strand as a template, the cell synthesizes a new strand . Meanwhile, the broken strand peels off, and one end enters the F– cell. There synthesis of its complementary strand begins. DNA replication continues in both donor and recipient cells, as the transferred plasmid strand moves farther into the recipient cell. Once DNA transfer and synthesis are completed, the plasmid in the recipi- ent cell circularizes. Now both cells are F+ cells.
46
What are ‘resistance genes’?
Genes that confer resistance to an antibiotic
47
What are genes that confer resistance to an antibiotic called?
‘Resistance genes'
48
What are resistance genes in the form of plasmids called?
R plasmids
49
What are ‘R plasmids’?
Plasmids that carry ‘resistance genes’ i.e. antibacterial resistance
50
What are plasmids that carry resistance genes called?
R plasmids
51
How can organisms be grouped based on their type of respiration? (think deeper than aerobic/anaerobic)
Obligate aerobes, obligate anaerobes and facultative anaerobes
52
What are ‘obligate aerobes’?
Organisms that must use O2 for respiration and thus can not survive with out it They can only perform aerobic respiration
53
What are ‘obligate aerobes’?
Organisms that can only perform anaerobic respiration. They are often poisoned by O2 due to its reducing nature.
54
What are some typical electron acceptors used in anaerobic respiration?
Nitrates (NO3-) and Sulfate (SO4 2-)
55
What are ‘facultative anaerobes’?
Organisms that use oxygen for aerobic respiration when possible. However they are also able to perform anaerobic respiration or fermentation when necessary in anaerobic conditions
56
What are organisms that only perform aerobic respiration called?
Obligate aerobes
57
What are organisms that only perform anaerobic respiration called?
Obligate anaerobes
58
What are organisms that can perform aerobic or anaerobic respiration called?
Facultative anaerobes
59
Which cells are notoriously facultative anaerobes in the human body?
Muscle cells
60
Based on the from of respiration they perform, how can muscle cells be classed?
Facultative anaerobes (they prefer to do aerobic, but can do anaerobic respiration if oxygen deprived)
61
What is it called when atmospheric nitrogen is converted into compounds?
Nitrogen fixation
62
What is nitrogen fixation?
When atmospheric nitrogen is converted into compounds i.e NH3
63
How can organisms be grouped based on nutritional modes?
Photoautotroph, chemoautotroph, photoheterotroph, chemoheterotroph
64
What are photoautrophs in terms of energy source and carbon source?
Energy Source: Light | Carbon Source: CO2, HCO3- etc.
65
What are chemoautotrophs in terms of energy source and carbon source?
Energy Source: Inorganic chemicals such as H2S, NH3 or Fe2+ | Carbon Source:CO2, HCO3- etc.
66
What are photoheterotrophs in terms of energy source and carbon source?
Energy Source: Light | Carbon Source: Organic compounds
67
What are chemoheterotrophs in terms of energy source and carbon source?
Energy Source: Organic compounds i.e glucose | Carbon Source: Organic compounds
68
What are are some examples of photoautotrophs?
``` Photosynthetic prokaryotes (for example, cyanobacteria); plants; certain protists (for example, algae) ```
69
What are are some examples of chemoautotrophs?
Unique to certain prokaryotes (for example, Sulfolobus)
70
What are are some examples of photoheterotrophs?
Unique to certain aquatic and salt-loving prokaryotes (for example, Rhodobacter, Chloroflexus)
71
What are are some examples of chemoheterotrophs?
Many prokaryotes (for example Clostridium) and protists; fungi; animals; some plants.
72
How do cyanobacteria perform nitrogen fixation?
Most cells in a filament carry out only photosynthesis, while a few specialized cells called heterocysts (sometimes called heterocytes) carry out only nitrogen fixation. Each heterocyst is surrounded by a thickened cell wall that restricts entry of O2 produced by neighboring photosynthetic cells. Intercellular connections allow heterocysts to transport fixed nitrogen to neighboring cells and to receive carbohydrates. This is important as the O2 produced in photosynthesis inhibits the enzymes used for nitrogen fixation
73
What are the basic places 'metabolic cooperation’ is seen?
Nitrogen fixation in cyanobacteria and in biofilms
74
What is ‘metabolic cooperation'
Cooperation between prokaryotic cells allows them to use environmental resources they could not use as individual cells.
75
What are heterocysts?
Specialised cyanobacteria that are specialised to perform nitrogen fixation. Note that they are the same species as the other cyanobacteria they metabolically cooperate with.
76
What are biofilms?
Sheets of bacteria that metabolically cooperate.
77
What are archaea?
They are one of the three basic domains but and are most similar to bacteria.
78
What are the basic ways that archaea, bacteria and eukarya can be compared?
Structure, Chemical composition, genetics and tolerance
79
How do bacteria, archaea and eukarya compare in terms of structure?
Bacteria, Archaea, Eukarya Nuclear envelope: absent, absent, present Membrane-bound organelles: absent, absent, present Circular chromosome: present, present, absent
80
How do bacteria, archaea and eukarya compare in terms of chemical composition?
Bacteria, Archaea, Eukarya Peptidoglycan in cell wall: present, absent, absent Membrane lipids: unbranched hydrocarbons, soem branched hydrocarbons, unbranched hydrocarbons RNA polymerase: one kind, several kinds, several kinds.
81
How do bacteria, archaea and eukarya compare in terms of genetics?
Bacteria, Archaea, Eukarya Initiator amino acid for protein synthesis: Formyl-methionine, methionine, methionine Introns in genes: very rate, present in some, common Histones associated with DNA: Absent, present in some species, present
82
How do bacteria, archaea and eukarya compare in terms of tolerance?
Bacteria, Archaea, Eukarya Response to the antibiotics streptomycine and chloramphenicol: Growth inhibited, no effect, no effect Growth at temperatures >100ºC: No, some species, no
83
What class of organism are all extremophiles?
Archaea
84
What are extremophiles?
Organisms (all archaea) that live in extreme environmental conditions
85
What are organisms that live in extreme environmental conditions called?
Extremophiles
86
What are the basic types of extremophiles?
Extreme halophiles and extreme thermophiles
87
What are extreme halophiles?
Those that live is extremely salty environments
88
What is the salinity of sea water?
3.5%
89
What are organism that live in very salty environments called?
Extreme halophiles
90
What are archae that release methane called?
Mathanogens
91
What are methanogens?
Archaea that release methane
92
What is a unique byproduct of respiration in some archaea?
Methane is produced by methanogens
93
What are the major groups of bacteria?
Proteobacteria, chlamydia, spirochetes, cyanobacteria and gram-positive bacteria
94
What are proteobacteria divided into?
Alpha proteobacteria, Beta proteobacteria, Gamma proteobacteria, Delta proteobacteria and Epsilon proteobacteria. (all are subgroups)
95
Describe proteobacteria.
Gram-negative bacteria that photoautotrophs, chemoautotrophs, and heterotrophs. Some proteobacteria are anaerobic, while others are aerobic.
96
What bacteria are gram-negative?
All groups except the ‘Gram-positive group'
97
What are alpha proteobacteria?
Many are associated with eukaryotic hosts For example Rhizobium (“root-nodule”) bacteria and Agrobacterium are alphas. Mitochondria evolved from aerobic alpha proteobacteria through endosymbiosis.
98
What class of bacteria are Rhizobacteria?
Alpha proteobacteria
99
What class of bacteria is Agrobacterium?
Alpha proteobacteria
100
What class of bacteria is mitochondria derived from?
Alpha proteobacteria
101
What are beta proteobacteria?
A nutritionally diverse subgroup that includes Nitrosomonas, a genus of soil bacteria that play an important role in nitrogen recycling by oxidizing ammonium (NH4+), producing nitrite (NO2–) as a waste product.
102
What are gamma proteobacteria?
A sub-group that includes some some bacteria. Many other members are pathogenic such as ’Salmonella’, ‘Legionella’ (cause Legionnaire’s disease ), Vibrio cholerae (cholera) and E. coli
103
What causes Legionaire’s disease?
Legionalla bacteria
104
What causes cholera?
Vibrio cholerae (a type of bacteria)
105
What does ‘Legionella’ bacteria cause?
Legionaire’s disease
106
What does ‘vibrio cholerae’ cause?
Cholera
107
What type of bacteria is Legionella?
Gamma proteobacteria
108
What type of bacteria is E. coli?
Gamma proteobacteria
109
What type of bacteria is salmonella?
Gamma proteobacteria
110
What type of bacteria is vibrio cholerae?
Gamma proteobacteria
111
What is a directed movement towards or away from a stimulus called?
A taxis
112
What is a positive taxis?
When the bacterium etc. moves towards a stimulus i.e. positive phototaxis towards light
113
What is the structure of a prokaryotic flagellum?
Embedded in the inner plasma membrane is a ‘motor’ which appears as a set of discs In the centre of these disks is a ‘rod’ which extends outside the cell wall. Just outside the cell wall the rod bends to form the ‘hook’. The ‘filament’ is the long trailing tail that extends form the hook
114
What is exaction?
When evolution repurposes features for other uses.
115
Where is the DNA of a bacterium located?
In a ’nucleoid'
116
What is a ’nucleoid’?
A region in a bacterium’s cytosol which holds the chromosome. Note that it is not membrane bound and is thus loosely defined. It appears lighter than surrounding areas in an electron microscope
117
What is a common way that bacteria tolerate changing environmental conditions i.e. periodic drought?
When they lack certain nutrients or during harsh conditions they can become dormant ‘endospores'
118
What are ‘endospores’?
A dormant form that many bacteria can assume when they lack certain nutrients or under harsh conditions. The original cell produces a copy of its chromosome and surrounds it with a tough multilayered structure, forming the endospore. Water is removed from the endospore, and its metabolism halts. The original cell then lyses, releasing the endospore. When dehydrated they become active bacteria
119
Does meiosis occur in prokaryotes?
Nope
120
What is the ploidy of prokaryotes?
They are all haploid
121
What is 'genetic recombination’?
The combination of DNA from two sources
122
How does ‘genetic recombination’ occur in eukaryotes?
With fertilisation and in meiosis (crossing over)
123
What is the combination of DNA form two sources called?
Genetic recombination
124
How does genetic recombination occur in prokaryotes?
Since they do not perform meiosis or fertilisation it only occurs in ‘horizontal gene transfer'
125
What are the basic methods of horizontal gene transfer?
Transformation, transduction, conjugation and plasmids
126
What is ’transformation’ as a method of horizontal gene transfer?
The genotype and possibly phenotype of a prokaryotic cell are altered by the uptake of foreign DNA from its surroundings. For example transformation occurs when a nonpathogenic cell takes up a piece of DNA carrying the allele for pathogenicity and replaces its own allele with the foreign allele, an exchange of homologous DNA segments. The cell is now a recombinant.
127
What is ’transduction’ as a method of horizontal gene transfer?
Phages (bacteriophages = viruses that infect bacteria) carry prokaryotic genes from one host cell to another. If some of this DNA is then incorporated into the recipient cell’s chromosome by DNA recombination, a recombinant cell is formed.
128
What is ’conjugation’ as a method of horizontal gene transfer?
DNA is transferred between two prokaryotic cells (usually of the same species) that are temporarily joined. In bacteria, the DNA transfer is always one-way: One cell donates the DNA, and the other receives it. The tow bacteria are held together by a pilus
129
What are ’plasmids’ as a method of horizontal gene transfer?
Plasmids are small sections of DNA. They can be transferred between bacteria in ‘horizontal gene transfer'
130
In what form of horizontal gene transfer are pili (singular: plies) involved?
Conjugation
131
How are certain forms of horizontal gene transfer regulated?
With the ‘F factor'
132
What are the forms of F factor?
It is just F factor if it exists in the bacterial chromosome. If it is in its own plasmid it is the ‘F plasmid'
133
What forms of horizontal gene transfer does the F factor regulate?
Conjugation and Plasmid
134
How does the F factor regulate conjugation?
The F factor consist of genes needed for the formation of a pilus etc. Cells that have the F factor are called HFR (high frequency recombinant) During conjugation they act as donors and transfer genetic information to the other bacterium. Note that the HFR bacterium can transfer the F factor to the host F- bacterium, causing it to become F+ (HFR)
135
What does the ‘F’ in F factor stand for?
Fertility
136
What is a ‘F+’cell?
One with the F factor
137
What is a ‘F-‘ cell?
One without the F factor
138
What does ‘HFR’ refer to?
High frequency recombination. It refers to a bacteria which has the F factor in its chromosome and thus is F+
139
How does can the F plasmid be transferred to another bacterium?
A cell carrying an F plasmid (an F+ cell) forms a mating bridge with an F– cell. One strand of the plasmid’s DNA breaks at one point. Using the unbroken strand as a template, the cell synthesizes a new strand . Meanwhile, the broken strand peels off, and one end enters the F– cell. There synthesis of its complementary strand begins. DNA replication continues in both donor and recipient cells, as the transferred plasmid strand moves farther into the recipient cell. Once DNA transfer and synthesis are completed, the plasmid in the recipi- ent cell circularizes. Now both cells are F+ cells.
140
What are ‘resistance genes’?
Genes that confer resistance to an antibiotic
141
What are genes that confer resistance to an antibiotic called?
‘Resistance genes'
142
What are resistance genes in the form of plasmids called?
R plasmids
143
What are ‘R plasmids’?
Plasmids that carry ‘resistance genes’ i.e. antibacterial resistance
144
What are plasmids that carry resistance genes called?
R plasmids
145
How can organisms be grouped based on their type of respiration? (think deeper than aerobic/anaerobic)
Obligate aerobes, obligate anaerobes and facultative anaerobes
146
What are ‘obligate aerobes’?
Organisms that must use O2 for respiration and thus can not survive with out it They can only perform aerobic respiration
147
What are ‘obligate aerobes’?
Organisms that can only perform anaerobic respiration. They are often poisoned by O2 due to its reducing nature.
148
What are some typical electron acceptors used in anaerobic respiration?
Nitrates (NO3-) and Sulfate (SO4 2-)
149
What are ‘facultative anaerobes’?
Organisms that use oxygen for aerobic respiration when possible. However they are also able to perform anaerobic respiration or fermentation when necessary in anaerobic conditions
150
What are organisms that only perform aerobic respiration called?
Obligate aerobes
151
What are organisms that only perform anaerobic respiration called?
Obligate anaerobes
152
What are organisms that can perform aerobic or anaerobic respiration called?
Facultative anaerobes
153
Which cells are notoriously facultative anaerobes in the human body?
Muscle cells
154
Based on the from of respiration they perform, how can muscle cells be classed?
Facultative anaerobes (they prefer to do aerobic, but can do anaerobic respiration if oxygen deprived)
155
What is it called when atmospheric nitrogen is converted into compounds?
Nitrogen fixation
156
What is nitrogen fixation?
When atmospheric nitrogen is converted into compounds i.e NH3
157
How can organisms be grouped based on nutritional modes?
Photoautotroph, chemoautotroph, photoheterotroph, chemoheterotroph
158
What are photoautrophs in terms of energy source and carbon source?
Energy Source: Light | Carbon Source: CO2, HCO3- etc.
159
What are chemoautotrophs in terms of energy source and carbon source?
Energy Source: Inorganic chemicals such as H2S, NH3 or Fe2+ | Carbon Source:CO2, HCO3- etc.
160
What are photoheterotrophs in terms of energy source and carbon source?
Energy Source: Light | Carbon Source: Organic compounds
161
What are chemoheterotrophs in terms of energy source and carbon source?
Energy Source: Organic compounds i.e glucose | Carbon Source: Organic compounds
162
What are are some examples of photoautotrophs?
``` Photosynthetic prokaryotes (for example, cyanobacteria); plants; certain protists (for example, algae) ```
163
What are are some examples of chemoautotrophs?
Unique to certain prokaryotes (for example, Sulfolobus)
164
What are are some examples of photoheterotrophs?
Unique to certain aquatic and salt-loving prokaryotes (for example, Rhodobacter, Chloroflexus)
165
What are are some examples of chemoheterotrophs?
Many prokaryotes (for example Clostridium) and protists; fungi; animals; some plants.
166
How do cyanobacteria perform nitrogen fixation?
Most cells in a filament carry out only photosynthesis, while a few specialized cells called heterocysts (sometimes called heterocytes) carry out only nitrogen fixation. Each heterocyst is surrounded by a thickened cell wall that restricts entry of O2 produced by neighboring photosynthetic cells. Intercellular connections allow heterocysts to transport fixed nitrogen to neighboring cells and to receive carbohydrates. This is important as the O2 produced in photosynthesis inhibits the enzymes used for nitrogen fixation
167
What are the basic places 'metabolic cooperation’ is seen?
Nitrogen fixation in cyanobacteria and in biofilms
168
What is ‘metabolic cooperation'
Cooperation between prokaryotic cells allows them to use environmental resources they could not use as individual cells.
169
What are heterocysts?
Specialised cyanobacteria that are specialised to perform nitrogen fixation. Note that they are the same species as the other cyanobacteria they metabolically cooperate with.
170
What are biofilms?
Sheets of bacteria that metabolically cooperate.
171
What are archaea?
They are one of the three basic domains but and are most similar to bacteria.
172
What are the basic ways that archaea, bacteria and eukarya can be compared?
Structure, Chemical composition, genetics and tolerance
173
How do bacteria, archaea and eukarya compare in terms of structure?
Bacteria, Archaea, Eukarya Nuclear envelope: absent, absent, present Membrane-bound organelles: absent, absent, present Circular chromosome: present, present, absent
174
How do bacteria, archaea and eukarya compare in terms of chemical composition?
Bacteria, Archaea, Eukarya Peptidoglycan in cell wall: present, absent, absent Membrane lipids: unbranched hydrocarbons, soem branched hydrocarbons, unbranched hydrocarbons RNA polymerase: one kind, several kinds, several kinds.
175
How do bacteria, archaea and eukarya compare in terms of genetics?
Bacteria, Archaea, Eukarya Initiator amino acid for protein synthesis: Formyl-methionine, methionine, methionine Introns in genes: very rate, present in some, common Histones associated with DNA: Absent, present in some species, present
176
How do bacteria, archaea and eukarya compare in terms of tolerance?
Bacteria, Archaea, Eukarya Response to the antibiotics streptomycine and chloramphenicol: Growth inhibited, no effect, no effect Growth at temperatures >100ºC: No, some species, no
177
What class of organism are all extremophiles?
Archaea
178
What are extremophiles?
Organisms (all archaea) that live in extreme environmental conditions
179
What are organisms that live in extreme environmental conditions called?
Extremophiles
180
What are the basic types of extremophiles?
Extreme halophiles and extreme thermophiles
181
What are extreme halophiles?
Those that live is extremely salty environments
182
What is the salinity of sea water?
3.5%
183
What are organism that live in very salty environments called?
Extreme halophiles
184
What are archae that release methane called?
Mathanogens
185
What are methanogens?
Archaea that release methane
186
What is a unique byproduct of respiration in some archaea?
Methane is produced by methanogens
187
What are the major groups of bacteria?
Proteobacteria, chlamydia, spirochetes, cyanobacteria and gram-positive bacteria
188
What are proteobacteria divided into?
Alpha proteobacteria, Beta proteobacteria, Gamma proteobacteria, Delta proteobacteria and Epsilon proteobacteria. (all are subgroups)
189
Describe proteobacteria.
Gram-negative bacteria that photoautotrophs, chemoautotrophs, and heterotrophs. Some proteobacteria are anaerobic, while others are aerobic.
190
What bacteria are gram-negative?
All groups except the ‘Gram-positive group'
191
What are alpha proteobacteria?
Many are associated with eukaryotic hosts For example Rhizobium (“root-nodule”) bacteria and Agrobacterium are alphas. Mitochondria evolved from aerobic alpha proteobacteria through endosymbiosis.
192
What class of bacteria are Rhizobacteria?
Alpha proteobacteria
193
What class of bacteria is Agrobacterium?
Alpha proteobacteria
194
What class of bacteria is mitochondria derived from?
Alpha proteobacteria
195
What are beta proteobacteria?
A nutritionally diverse subgroup that includes Nitrosomonas, a genus of soil bacteria that play an important role in nitrogen recycling by oxidizing ammonium (NH4+), producing nitrite (NO2–) as a waste product.
196
What are gamma proteobacteria?
A sub-group that includes some some bacteria. Many other members are pathogenic such as ’Salmonella’, ‘Legionella’ (cause Legionnaire’s disease ), Vibrio cholerae (cholera) and E. coli
197
What causes Legionaire’s disease?
Legionalla bacteria
198
What causes cholera?
Vibrio cholerae (a type of bacteria)
199
What does ‘Legionella’ bacteria cause?
Legionaire’s disease
200
What does ‘vibrio cholerae’ cause?
Cholera
201
What type of bacteria is Legionella?
Gamma proteobacteria
202
What type of bacteria is E. coli?
Gamma proteobacteria
203
What type of bacteria is salmonella?
Gamma proteobacteria
204
What type of bacteria is vibrio cholerae?
Gamma proteobacteria
205
What are photosynthetic bacteria classed as?
Cyanobacteria
206
Describe bacteria in the chlamydia group.
These parasites can survive only within animal cells, depending on their hosts for resources as basic as ATP. The gram-negative walls of chlamydias are unusual in that they lack peptidoglycan
207
Describe spirochete bacteria?
They are helical (spiral shaped) heterotrophs Many are free living but a few are parasistes
208
What is ‘phyloplankton’?
The collection of photosynthetic organisms that drift near the water's surface. It includes organism of many types, including cyanobacteia
209
What are bacteria etc. that cause disease called?
Pathogens
210
What are pathogens?
Bacteria etc. that can cause disease
211
What does ’pathogenic bacteria’ refer to?
Bacteria that cause disease
212
Why is vibrio cholerae so harmful?
It releases an exotoxin that stimulates intestinal cells to release chloride ions into the gut, and water follows by osmosis.
213
What are harmful substance released by bacteria called?
Toxins
214
What are toxins?
Harmful substance released by bacteria etc.
215
What are toxins released by bacteria divided into?
Exotoxins and endotoxins
216
What are exotoxins?
Proteins secreted by certain bacteria and other organisms that cause harm
217
What are endotoxins?
Lipopolysaccharide components of the outer membrane of gram-negative bacteria. They are released only when the bacteria die and their cell walls break down.
218
Is salmonella caused by an endotoxin or an exotoxin?
An endotoxin
219
What is an example of an endotoxin?
The one that causes salmonella poisoning
220
What is the use of organisms to remove pollutants from an environment called?
Bioremediation
221
What is bioremediation?
The use of organisms (including bacteria) to remove pollutants from soil, air, or water.