2nd Credit Test - Deck 2 Flashcards
(15 cards)
The results of immunofluorescence tests are evaluated by the help of:
Fluorescence microscope.
The positive result in complement fixation test (CFT) is:
Inhibition of lysis of sensitised erythrocytes.
Based on the presence of CD markers is possible to separate leukocytes by:
Flow cytometry (FACS) or magnetic separation.
Forward scatter in flow cytometer detects:
Size of cells.
For lysis of erythrocytes in procedure of separation of all leukocytes from whole blood, blood must be incubated with hypotonic solution for:
35-40 seconds.
Serum in which we want to determine the titer of antibodies by complement fixation test:
Has to be treated at 56°C for 30 minutes.
For evaluation of the microbicidal activity of the phagocytes, the heparinised whole blood is incubated one hour with:
Live bacteria or yeast.
The index of phagocytic activity we can calculate as:
The average number of particles per one phagocytic cell.
If you counted 78 leukocytes in 50 squares in Bürker chamber, the number of leukocytes in 1 ml of blood is:
7.8 x 10⁶/ml
The concentration of leukocytes in the blood of different animals is:
5-10 x 10⁶/ml
For testing respiratory burst in phagocytes, we use phorbol myristate acetate (PBA) because:
Activates respiratory burst directly.
For evaluation of phagocytic activity and index of phagocytic activity we can use:
Ingestion of different particles, e.g. latex particles, killed yeast (e.g. C. albicans), zymosan.
The ratio blood : H2O : 2.7% NaCl used for lysis of erythrocytes in the procedure of separation of all leukocytes from whole blood is:
1:2:1
In flow cytometry the following is detected:
Size and granularity.
Amboceptor is ?
Rabbit anti-Ab’s against Sheep erythrocytes.
