3.1: Methods Overview

Question 1

sample complexity: cellular vs isolated protein
different factors

Answer 1

background contributions - stronger for cellular
ability to identify - difficult for cellular
resolution - lower and limited for cellular

Question 2

Protein behaviour

Answer 2

dynamic and often have more than 1 functional state which is difficult to control

Question 3

how to deal with contrast and what is the issue

Answer 3

Higher dose = inc imaging but also causes radiation damage

> need to balance

Question 4

foundation of contrast

Answer 4

Biomolecules usually have light molecules and require aq. environment
Imaging with light/e-/x-rays = little contrast between bg and protein
Low signal:noise ratio

Question 5

X-ray crystallography basics abt method n growth

Answer 5

most commonly used method, linear growth

Question 6

NMR growth

Answer 6

less common and declining growth

Question 7

EM (e- microscopy) growth

Answer 7

accelerating growth