bunya Flashcards
What’s unique about bunyaviridae envelopes?
there’s no matrix protein
describe bunyaviridae genomes
SEGMENTED, negative sense, ssRNA
bunyaviridae are generally ______ for vertebrate cells
cytocidal
what are the four genera of bunyaviridae?
arthropod borne:-orthobunyavirus; akabane-phlebovirus; rift valley fever-nairovirus; Nairobi sheep virusnon-arthropod borne:-hantavirus; Hantaan
etiology of Rift Valley Fever virus?
phlebovirus, family bunvyaviridae
hosts of Rift Valley Fever?
sheep cattle and goatscan infect humans as well
how does rift valley infect humans?
percutaneous or aerosol exposure while slaughtering or handling infected fetusAedes mosquito bitesNO HUMAN TO HUMAN TRANSMISSION
rift valley fever occurs throughout _________
most of africa
main vectors of rift valley fever?
mosquitos(biological):Primary - Aedes sppSecondary - Culex spp, and Anopheles sppbiting flies(mechanical) - culicoides
describe the epizootic-endemic cycle of rift valley fever
-flooding of dambos (increased rain)-massive emergence of transovarially infected Aedes –> feed on livestock –> abortion storms(epizootic) –> culex/anopheles infections –> human infections (epidemic)details: The virus is transmitted transovarially (thorugh egg) among floodwater Aedesspp. mosquitoes. The virus survives for very long periods in mosquito eggs laid at the edges of usually dry depressions, called “dambos”. When the rains come and the dambos flood, the eggs hatch, and infected mosquitoes emerge and infect nearby wild and domestic animals. These Aedesmosquitoes become very numerous after heavy rains, and infect sheep and cattle. Large number of these infected sheep and cattle become viremic, allowing many more mosquitoes (inducing secondary mosquito vectors) to become infected. This amplification, together with mechanical transmission by biting flies, results in infection and disease in a very high proportion of animals and humans at risk
describe the pathogenesis of rift valley fever
Rift Valley fever virus replicates rapidly and reaches very high titer in target tissues. Virus replicates in liver and other major organs, resulting in widespread cell necrosis. Extensive hepatocellular necrosis is common in terminally affected sheep. Encephalitis, hemorrhages in GI tract and enlarged spleen. Hepatic necrosis, renal failure, and shock, sometimes with hemorrhagic complications, are the primary causes of death. About 90-100% of pregnant animals abort
clinical signs of rift valley fever?
Sheep: Mortality in young lambs is high (90%–100%) Young animals surviving the hepatic infection may show encephalomyelitis. 90-100% of pregnant ewes abort. Cattle: Less severe than sheep. However, 90-100% of infected pregnant cows abort
rift valley fever Dx?
RVF should be suspected when abnormally heavy rains and flooding are followed by widespread occurrence of abortions and mortality among newborn animals characterized by necrotic hepatitis, concurrent with influenza-like disease in people handling animals or their products
rift valley fever control?
Control is based primarily on livestock vaccination, but vector control (via use of mosquito larvicides and insecticides) is also used during outbreaks. Attenuated-virus Rift Valley fever vaccines produced in mouse brain and in embryonated eggs are effective and inexpensive for use in sheep, but they cause abortions in pregnant ewes. Inactivated-virus vaccines produced in cell cultures avoid the problem of abortion, but are expensive
Akabane is a ________-transmitted virus that causes ___________________ in ruminants
insect; congenital abnormalities of the CNS