T cell transcription factors

Question 1

How does the CD4/8 transduce the signal?

Answer 1

Lkc associated tyrosine kinase in the cytosol phosphorylates ITAMs on the ZETAs: this provides a binding site for ZAP-70

ZAP-70 binds, is phosphorylated by Lck
ZAP-70-P is now a binding site for LATs, which phosphorylate PLC initiates downstream signaling

Question 2

PLC –>

Answer 2

IP3 —————> PKC ——-> IkB inactivation —> NFkB activation —> IL-2 gene

Question 3

PKC —>

Answer 3

inactivates IkB, which in turn releases NFkB

Question 4

NFkB —>

Answer 4

transcription factor that activates IL-2 gene

Question 5

RAS and MAP —>

Answer 5

AP-1 activation via Ca influx

Question 6

AP-1

Answer 6

transcription factor to synthesize IL-2

Question 7

NFkB —>

Answer 7

IL-2 synthesis

activated by PKC, which inactivates IkB, which is iniaited by PLC

Question 8

AP-1 –>

Answer 8

IL-2 synthesis

activated by Ras-MAP PWs

Question 9

How is IkB inactivated?

Answer 9

ubiquination

Question 10

Signal 2

Answer 10

Cd28–CD80/86 T cell – APC

Question 11

Signal 1 w/out costimulation

Answer 11

APC–TCR+No Co Stim –> NFAT (transcription factor)—> Anargy inducing signal

Question 12

Signal 1 w/costimulation

Answer 12

Signal 1 (TCR) + Signal 2 (Costim)

Sig 1 –> NFkB
Sig 2 –> NFAT

NFAT + NFkB –> reproductive response

Question 13

P13, PDK1, and AKT in T cell activation: first, what’s the point?

Answer 13

the point is inactivating apoptotic proteins

Question 14

P13, PDK1, and AKT in T cell activation: how does it work?

Answer 14

It works via P13 kinase: converts PIP2 –> PIP3

PIP3 activates PDK-1: PDK activates AKt

Question 15

P13, PDK1, and AKT in T cell activation: the “train” of events superficially

Answer 15

P13K activates PIP2 –> PIP3 –> PDK-1 –> Akt –> cell survival

Question 16

P13, PDK1, and AKT in T cell activation: what are the last two proteins involved?

Answer 16

PDK-1 —> Akt

CELL SURVIVAL