4.2 flagella structure Flashcards
(21 cards)
the flagellum. is what type of structure
polymeric – built in orderly fashion
flagella has three distinct components composed of different proteins
basal body, rod and hook, and filament.
basal body location
located in the cell envelope, the inner structures of the rotor
– include C ring, MS ring, P ring, and L ring. – rings act like bushings
surrounding the rotor are the..
stators
what do stators do
anchor the structure into the cell membrane at the peptidoglycan layer.
stators are a site where
ions flow from inside of the cell to outside
rod and hook located
anchored to cell envelope but external to cell -
rod is attached to what
P and L rings and pokes out through the outer membrane – the hook is attached to the rod
filament location
completely extracellular, most abundant protein content of entire structure
– long hair-like part we can see using staining
type III secretion system
includes the basal body, rod, and hook. – a system for secretion of components of the flagellum.
proeins must be available for assembly in..
the correct order to make construction process smooth.
basal body must be built..
before the rod and hook can be assembled.
rod and hook must be in place..
before the filament can be constructed.
the phenotype of wild cells is..
large colonies
phenotype is what researchers can..
see
genotype indicates
what genes are mutant in. the strain
what does the delta sign in a gene indicate
that the gene is deleted.
what does it mean when gene is deleted
means colony is small, and has lost the ability to make flagella.
each gene deletion is complimented..
by the gene that was deleted ‘C’
what do the complement strains form
large colonies – therefore ability to make flagella.
examples of deleted and complemented genes
deleted:
D = delta
DflhB, DfliM, DfliY
C = complement
CDflhB, CDfliM, CDfliY
