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Microbiology Module 1 '15 - '16 > Introduction to Microbiology Techniques > Flashcards

Introduction to Microbiology Techniques Flashcards

1
Q

What is a defined media?

A

Media where all ingredients AND their amounts are known.

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2
Q

What is another name for a Primary media, what does it do and what are examples?

A

Non selective media, supports large number of organisms. Sheep Blood agar, Trypticase soy

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3
Q

What is selective media and what is an example?

A

Inhibits some organisms, CNA

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4
Q

What is differential media and what is an example?

A

Indicators of biochemical reactions, MAC

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5
Q

What are some examples of combination medias?

A

MAC, XLD, HEK

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6
Q

What are enrichment medias? Examples?

A

Allows for more growth than primary media, Chocolate. Often incubated in CO2 for fastidious organisms.

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7
Q

What are maintenance medias? What can be grown on them?

A

Allows for extended growth time (limits sugars or adds extra buffer) Salmonella and E. Coli.

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8
Q

What do Dyes inhibit?

A

Gram pos

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9
Q

What are some selective agents and what do they inhibit?

A 
Heavy Metals (ex: Bismuth sulfite for Salmonella*)
Antimicrobials
Sodium desoxycholate (bile salts) inhibits GP
Phenylethyl alcohol= inhibits GN
Azide= Inhibits GN
NaCl = Only allows Staphylococcus
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10
Q

What type of media is SBA?

A

Primary

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11
Q

What type of media is Trypticase Soy?

A

Primary

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12
Q

What type of media is CNA?

A

Selective

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13
Q

What type of media is MAC? Selective for? Colors?

A

Selective and differential; selective for Gram (-), dark pink = pos= uses lactose Clear = (bright pink)= neg= does not use lactose

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14
Q

What are specimens from normally sterile areas place on?

A

Enrichment (chocolate)

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15
Q

What things are taken into account when selecting a media?

A

Type of specimen
What condition is suspected
Results of preliminary exam of specimen

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16
Q

What type of Media is XLD? selective for? Colors?

A

Selective Isolation and Selective differential media; Selects Gram (-) via bile salts; CHO (+)= Yellow, CHO (-) = Clear/red H2S = Black

17
Q

What type of media is HEK?

A

Hektoen; Selective Isolation and Selective differential media Selects Gram (-) via bile saltsCHO (+)= orange, CHO (-) = Clear/gree H2S = Black

18
Q

What type of media is EMB?

A

Selective Isolation and Selective differential media Selects Gram (-) Ecoli = metallic green

19
Q

What type of media is bismuth sulfide?

A

Heavy metals; Selective FOR Gram (-)

20
Q

What type of media is thoglycollate?

A

liquid; anaerobes grow lower

21
Q

What media type is Brain Heart infusion?

A

Primary

22
Q

What media type is Martian lewis or Thayer Martian? What grows on it?

A

Selective enrichment agar for fastidious, inhibits usually flora for Neisseria gonorrhoea

23
Q

What are the three levels of hemolysis?

A 
Alpha = Partial and Green
Beta = Complete
Gamma = no hemolysis
24
Q

What is the most commonly used disinfectant used on non metallic surfaces?

A

10% Bleach

25
Q

What is the standard temperature, PSI, and time for autoclave?

A

121 Degrees C, 15PSI, 15-20min

26
Q

What is Obligate anaerobe

A

Requires O2

27
Q

What is it called when an organism requires little O2?

A

Microareophilie

28
Q

What is a facultative anaerobe?

A

Grows best with O2 but can grow without O2

29
Q

What is Ambient Air and its Element amounts?

A

Room air, non-CO2, O2=21% CO2= .03%

30
Q

What are CO2 incubator element amounts? What kind of incubator is it?

A

O2= 15% CO2= 5-10%; aerobic

31
Q

What are Microareophilic incubator elements?

A

O2= 5% CO2= 10%

32
Q

What are anaerobic incubator elements?

A

O2=0% CO2= 5-10% (rest is N)

33
Q

What goes in ambient air incubation?

A

Biochemicals, differential media, Antimicrobial susceptibility

34
Q

What goes in CO2 incubation?

A

Primary plates and fastidious organisms

35
Q

What is a direct smear?

A

Specimen is plated directly on slide and then gram stained provides initial clue to cause of infection

36
Q

What are two stains that require fluorescent microscope readings?

A

Acid fast, Acridine orange, and Calcofluor white

37
Q

What is the most important characters for making the diagnosis of “gangrene” based on a direct smear?

A

Necrotic Background and absence of intact cells

38
Q

What method is MALDI-TOF?

A

Rapid method

Microbiology Module 1 '15 - '16 (9 decks)

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