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Microbiology Module 1 '15 - '16 > Biochemicals > Flashcards

Biochemicals Flashcards

1
Q

What is the principle of the Methyl Red test?

A

If the organism can produce acid/neutral products via Glucose Fermentation.

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2
Q

What is the principle of the Voges-Proskauer test?

A

IF the product is Acetone from glucose fermentation

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3
Q

What is the principle of the Citrate test?

A

If the organism can use Citrate as its sole source of carbon.

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4
Q

What is the Principe of the Phenylalanine test?

A

Ability to deaminate Phenylalanine to Phenylpyruvic acid

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5
Q

What is the principle of the Indole test?

A

Spit indole from amino acid Tryprophane

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6
Q

What is the principle of the KIA test?

A

Ability to ferment lactose and/or glucose with/without Gas production as well as H2S

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7
Q

What is the principle of the motility test?

A

If an organism is motile (flagellated) or non-motile (non-flagellated)

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8
Q

What is the principle of the Urease test?

A

Able to split Urea via Urease

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9
Q

What is the principle of the Decarboxylase Dihydrolase test?

A

Presence of decarboxylase enzymes to break down Amino Acids to their Amine and CO2

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10
Q

What is the principle of the Arginine Dihyrolase test?

A

If an organic has arginine dihydrolase to break down arginine

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11
Q

What does a positive and negative mean in the methyl red test?

A

Positive used Mixed acid fermentation

Negative used Butylene Glycol

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12
Q

What fermentation do positive VP organisms use?

A

Butylene glycol fermentation

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13
Q

What is another name for the the phenylalanine test?

A

TDA

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14
Q

What are the two procedures of the Indole test?

A

Ehrlich and Kovacs

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15
Q

What is the advantage of one procedure over the other in the Indole test?

A

Ehrlich is more sensitive than Kovac

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16
Q

What is the Ehrlich procedure used for?

A

GNR and anarobes

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17
Q

What is the Kovacs procedure used for?

A

Enterobacteriaceae

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18
Q

What is the substrate and product of the KIA test?

A

Sodium Thiosulfate + H2S –> Black ppt. which is Ferrous Sulfide.

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19
Q

What is the difference in sugars among the Kia medias?

A

There is 10x more lactose than glucose and TSI has an additional 1% more sucrose

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20
Q

What can be added to the motility test, why? What is its disadvantage?

A

Tetrazolium Salts which help to visualize motility but can be inhibitory

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21
Q

What is the media used in urease? what is special about it?

A

Christens media and Proteus is strongly positive

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22
Q

What are two amino acids broken down in the Dacarb test and what are they broken down to?

A

Lysine to cadaverine and Ornithine to Putrescine

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23
Q

What is the substrate and reagent of the methyl red?

A

Glucose and methyl red

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24
Q

What is the substrate and reagent of the VP test?

A

Glucose; alpha naphthol AND KOH

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25
Q

What is the substrate and reagent of the Citrate test?

A

Citarte and NONE

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26
Q

What is the substrate and reagent of the Phenylalanine test?

A

Phenylalanine and FeCl3

27
Q

What is the reagent used in the indole test?

A

P-dimethylaminobenzaldehyde-HCl

28
Q

What is the substrate and reagent of the motility test?

A

None and NONE

29
Q

What is the substrate and reagent of the Urease test?

A

Urea and NONE

30
Q

What is the substrate and reagent of the decarboxylase dihyrolase test?

A

Lysine or ornithine and none

31
Q

What is the substrate and reagent of the arginine dihyrolase test?

A

Argine and none

32
Q

What is a positive and negative of the methyl red test?

A

Red is (+), Yellow is (-)

33
Q

What is a positive and negative of the VP test?

A

Pink is (+), Yellow is (-)

34
Q

What is a positive and negative of the Citrate test?

A

Blue is (+), Green is (-)

35
Q

What is a positive and negative of the Phenylalanine test?

A

Dark Green is (+), Yellow is (-)

36
Q

What is a positive and negative of the Indole test?

A

Red line in middle is (+) for Ehrich on the top is (+) for Kovac, Yellow is (-)

37
Q

What is a positive and negative of the KIA test?

A

A/A is Glucose and lactose (Yellow/Yellow); K/A is only glucose (Pink/yellow) blacking with Pink top is assumed to have been glucose only and H2S (+) K/K is NONE (pink/pink)

38
Q

What is a positive and negative of the Motility test?

A

Fanned/turbid is (+), single line is (-)

39
Q

What is a positive and negative of the Urease tests?

A

Pink is (+), Yellow is (-)

40
Q

What is a positive and negative of the Decarboxylase dihyrdolase test?

A

Purple/brown is (+), Yellow is (-)

41
Q

What is a positive and negative of the Arginine dihydrolase test?

A

Purple/brown is (+), Yellow is (-)

42
Q

What two tests should not be read early?

A

KIA and the decarboxylase

43
Q

What is the principle of the carbohydrate 1% test?

A

Degrade carbohydrates to produce acid as well as gas or not

44
Q

What is the principle of the Oxidation Fermentation test?

A

Utilization of glucose either fermentative, oxidative or neither

45
Q

What is the principle of the Cytochrome Oxidase test?

A

Presence of oxidized cytochrome C

46
Q

What is the principle of the Nitrate test?

A

Reduce nitrate to nitrite OR nitrate to N2 gas

47
Q

What is the principle of the MUG test?

A

Looking for Beta-glucuronidase (in e.coli)

48
Q

What is the broth and indicator in the 1% carbohydrate test?

A

Purple Broth base, bromcresol Purple indicator

49
Q

What is the tube system called in OF, what is in it?

A

Hugh and Ceifson’s, High Carbohydrate and Low peptones

50
Q

How do you inoculate the oxidase test?

A

NEVER nichrome loops, wooden applicator

51
Q

What is the medium with the nirate test?

A

Potassium nitrite

52
Q

What is the full name of the MUG test?

A

4-methylumbelliferyl-Beta-P-Glucurdase

53
Q

What is the substrate and reagent of the 1% carbohydrate test?

A

1!!! Carb and NONE

54
Q

What is the substrate and reagent of the OF test?

A

Glucose and NONE

55
Q

What is the substrate and reagent of the Cytochrome Oxidase test?

A

(filter paper) and 1% tetamethly-P-phenylenediaminedihyro chloride

56
Q

What is the substrate and reagent of the Nitate test?

A

Nitrate and first (alpha naphylamine, sulfonic acid) then if neg, zinc dust

57
Q

What is the substrate and reagent for the MUG test?

A

rapid test

58
Q

What is a positive and negative of the 1% carbohydrate test?

A

Yellow (+) and Purple (-)

59
Q

What is a positive and negative of the OF test?

A

Yellow in both = fermenter OR yellow in only oil= Oxidizer OR neither yellow (both green/red) = Asaccharolytic (non-oxidizer)

60
Q

What is a positive and negative of the Oxidase test?

A

Purple/blue (+), no change (-)

61
Q

What is a positive and negative of the Nitrate test?

A

Red (+) with first clear means you must add ZINC dust THEN RED (-) and CLEAR is (+)

62
Q

What is a positive and negative of the MUG test?

A

Florescent (+) no florescence (-)

63
Q

What is the main advantage of performing the Lead acetate test?

A

If is more sensitive for H2S than the KIA test is.

Microbiology Module 1 '15 - '16 (9 decks)

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