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Microbiology > entero-gram negative > Flashcards

entero-gram negative Flashcards

1
Q

serological test identifies grp A strep through

A

latex agglutination/nucleic acid probe

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2
Q

rgt for catalase test

rxn to enterecocci

A

3% hydrogen peroxide

(-) no bubble formation

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3
Q

LAP means

A

Leucine aminopeptidase test

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4
Q

antigen detection

A

serological test

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5
Q

drug of choice for penicillin-resistant n.gonorrhea

A

ceftriaxone

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6
Q

differentiates aerococcus and leuonostoc to other g+ cocci

A

lap test

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7
Q

appearance of moraxella catarrhalis in culture

A

hockey puck appearance

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8
Q

young colonies of s.pneumoniae

A

dome-shaped
glistening
wet
mucoid

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9
Q

uses antibody bound to a particle to enhance visibility of agglutination rxn between Ag and Ab

A

co-agglutination test

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10
Q

modt common isolate inchildren with recurrent otitis media

A

s. pneumoniae

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11
Q

test which detects acetoin production from glucose

A

voges-proskauer test

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15
Q

virulence factor of s. pnuemoniae

A

capsular polysaccharide

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17
Q

present in martin lewis medium

A

all in modified TMA except nystatin, which is replaved by anisomycin: yeast

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18
Q

(+) for voges proskauer test

end product ?

A

anginosus grp

acetoin (red/pink color)

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19
Q

appear as satellites around an organism that produces pyridoxal

A

abiotrophia and granulicatella

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20
Q

s. pnemoniae in microscope

A

gram-positive cocci in pairs, oval or lancet shape, faculatatively anaerobe

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21
Q

(-) to LAP test

A

aerococci

leuconostoc

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22
Q

specimens used in lab diagnosis for s. pneumonie

A

sputum
blood
csf

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23
Q

growth factors of abiotrophia and granulicatella

A

thiol compounds (cystein,vit b6,pyridoxal)

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24
Q

most commonly isolated member of genus moraxella

A

moraxella catarrhalis

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25
Q

old colonies of s.pneumoniae

A

coin w/ raised rim

dimple/donut-shaped

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26
Q

gram stain: s. pneumoniae

A

direct smearsof csf reveal wbcsand gram+ cocci in pairs

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28
Q

3 differential tests for s. pneumoniae

A

optochin susceptibility/p disk test
bile solubility
neufeld quellung rxn

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30
Q

(neufeld quellung rxn)

this is mixed with thesputum,csf,etc along with methylen blue

A

antipneumococcal serum

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31
Q

opsonized particles are more easily phagocytosed dueto presence of ___ on plasma membrane

A

complement receptors

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34
Q 
*s.pneumoniae
presumptive test?
rgt used?
susceptibility medium?
result?
A

optochin susceptibility/p disk test
ethylhydroxycuprein hydrochloride (6 or 10 um)
BAP
greater or equal to 14 mm

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36
Q

differentiates pneumococcus from viridans strep

A

bile solubility

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37
Q

compliction of pneumonia and otitis meia

A

meningitis

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38
Q

(+) result of m.catarrhalis in butyrate esterase test

A

blue color

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39
Q

(+)result of neufeld quellung rxn

A

swellingof capsule

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40
Q

formerly known as nutritionally variant streptococci

A

abiotrophia

granulicatella

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41
Q

appearance of neisseria in culture

A

small, gray-white opaque,convex, glistening colonies

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42
Q

capsule of s.pneumoniae is

A

antigenic

43
Q

abiotrophia and granulicatella are(3)

A

pyridoxal/vit b6-dependent
thiol-dependent
symbiotic streptococci

44
Q

opsonization of the capsule of s.pneumoniae renders the organism

A

avirulent

45
Q

3rd most common cause of otitis media and sinusitis

A

moraxella catarrhalis

46
Q

give at least 7 characteristics of neisseria

A 
obligate aerobic
non motile
non hemolytic
capnophiles (2-8%)
sensitive to drying and extreme heat
carbohydrate fermenters
fastidious; enriched medium is used
47
Q

bestmethod for culture andtransport of n.gonorrhea is

A

inoculate the agar immediately and place the medium in an increased CO2 such as coplin jar, JEMBEC system

48
Q

culture media for meningitidis (3)

A

bap
cap
tma

49
Q

neisseria observed in microscope

rod shaped?

A

gram-negative diplococci with coffee or kidney bean shaped

N. elongata, N. weaver

50
Q

only member of family neisseriaceae which is (+) on DNAse test

A

m. catarrhalis

51
Q

natural habitat of neisseria

A

mucuos membrane of respiratory & urogenital tract

52
Q

n.menigitidis is sensitive to ___; this should not exceed ___

A

sps; 0.025%

53
Q

beta lactamase test?
rgt?
(+) result?

A

cephalosporinase test
cephalosporin disk
deep pink

54
Q

gram variable and pleomorphic in microscope

A

abiotrophia

granulicatella

55
Q

*s.pneumoniae
confirmatory test?
rgt used?
result?

A

bile solubility
sodium desoxycholate(bile salt)
(+) bile soluble, (-) turbid

57
Q

biochemical identification of n.gonorrhea

(+) result?

A

coagglutination

agglutination

61
Q

major pathogens of neisseria (2)

A

n. gonorrhea

n. meningitidis

62
Q

presence of n.menigitidis in blood

A

meningococcemia

63
Q

infection shows bloody, rust stinged sputum

A

lobar pneumonitis

64
Q

other name for n. gonorrhea

only known host

A

gonococci

man

65
Q

n. gonorrhea requires __ for growth
it produces ___ which is important for pathogenesis
virulence factor?(for adherence)

A

iron
igA protease
common pili

66
Q

colonial typesof n.gonorrhea:
virulent?
avirulent?

A

t1 t2

t3 t5

67
Q

present in new york city medium

A

vancomycin
colistin
trimethoprim
amphotericin B: yeast

68
Q

in n.gonorrhea, these rare isolates are isolated from asymptomatic men

A

auxotypes

70
Q

(n.meningitidis)
clsi recommends what broth? and what agar microdilution?
with?

A

MH broth with 2-5% laked horse blood

MHA with 5% sheep blood

72
Q

AHU stains (3)

A

arginine
hypoxanthine
uracil

73
Q

means “flow of seed” and “brothel”

A

gonorrhea

74
Q

endotoxin produces hemorrhage in adrenals, which causes what syndrome

A

waterhouse-friderichsen syndrome

75
Q

other name for conjuctivitis

A

opthalmia neonatorum

76
Q

chief complaint oropharyngeal infection

A

pharyngitis

79
Q

specimens used for n.gonorrhea

A

pus and secretions from cervix,urethra, throat,prostate

80
Q

specimen collection for n.gonorrhea

then place inwhat transport medium? how long?

A

dacron or rayon swabs

amies medium for 6 hrs

81
Q

screening test for n.gonorrhea

A

nucleic acid amplification

83
Q

JEMBEC means

A

james e. martin biological environmental chamber

84
Q

confirmation of n.gonorrhea is based on (2)

A

growth and biochemical characteristics

85
Q

uses monoclonal bodies that recognizes epitope;

also confirms morphologic appearance

A

fluorescent antibody test/FAT

86
Q

antimicrobial agents present in modified TMA, suppressed organism

A

all in TMA

trimethoprim plate: swarming of proteus

87
Q

pyridoxal producers (4)

A

e.coli
kliebsiella spp
enterobacter spp
yeast

88
Q

virulence factors of n.meningitidis (4)

A

common pili
capsular polysaccharide
endotoxin
igA1

89
Q

confirmatory test for n.gonorrhea

A

chemiluminescent nucleic acid probe

90
Q

present in GC-LECT medium

A

all in NYC medium

lincomycin: g+

91
Q

other bacteria with (+) growth on gonococcal media (3)

A

acinetobacter
capnocytophaga
k.denitrificans

92
Q

standard method in identifying n.gonorrhea
detects?
medium?
(+) result?

A

CHO utilization test
acid production from glucose, maltose, lactose, fructose
cystine trypticase agar
yellow color at 35-37C

93
Q

rgt used for oxidase test in neisseria

(+) result?

A

1% tetramethyl-p-phenylenediamine dihydrochloride

purple color within 10 sec

95
Q

s. pnuemoniae is also known as

A

diplococcus/pneumococcus

99
Q

culture media for n.gonorrhea

if swabs, what pattern?

A

bap & cap

z-pattern and cross streaked with loop

104
Q

antimicrobial agents present in TMA (3); and their suppressed organism

A

vancomycin: g+
colistin: g-
nystatin: yeast

106
Q

staph and strep are motile, T/F

A

F, brownian movement

112
Q

untreated gonococcal cerviitis may cause

A

fitz-hugh-curtis synrome

138
Q

(+) to LAP test?

end product?

A

viridans streptococci
stretococcus
enterococcus
pediococcus

B-naphthylamine (red color)

Microbiology (6 decks)

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