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Microbiology > staph & strep (lab) > Flashcards

staph & strep (lab) Flashcards

1
Q

most commonly isolated viridans strep

A

s.mutans

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2
Q

test which detects unbound coagulase?
(+) result within?
screening?
confirmation?

A

tube method
clot formation within 4 hrs
1-4 hrs
20 hrs

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3
Q

culture: BAP staph

A

buttery looking/ creamy,white or light gold

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4
Q 
staph
2 coagulase (-) species
A

s. epidermidis

s. saprophyticus

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5
Q

bacitacin test or?
units?
to differentiate?
which is susceptible/resistant?

A

taxo A disk tes
0.04 units
micrococci (s; >10mm zone of inhibition) & staph (r)

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6
Q

differentiates patho and nonpatho staph?
(+) result? in what organism?
agar used?
composed of?

A

mannitol fermentation test
yellow; s.aureus
MSA
1%mannitol and 7.5% NaCl

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7
Q

most common isolated enterococci

A

e.faecalis

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8
Q

test which detects dnase activity of s.aureus?
medium and rgt used?
(+) result?

A

DNAse test
DNA medium; methyl green dye
clearing of dye

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9
Q

rgt for coagulase test

A

edta plasma

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10
Q

staph
normal flora of skin?
cause what diseases? (2)

A

s.epidermidis

endocarditis, prostethic 💟 valve

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11
Q

test for identification of s. saprophyticus?

zone of inhibition?

A

5 ug novobiocin: resistant

6-12 mm

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13
Q

grp which is only susceptible to bacitracin

A

A

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14
Q

s.epidermidis is ___ to novobiocin?

zone of inhibition?

A

susceptible

16-27mm

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15
Q

coagulase (-) staph by tube method

A

s.lugdunensis

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16
Q

characteristics of strep (5)

A 
catalase (-)
fastidious
pairs, chains
facultative anaerobe
capnophilic
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17
Q

alpha hemolytic strep

A

s.pneumoniae

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18
Q

appearance in microscope (staph)

A

spherical in clusters

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19
Q

beta hemolytic strep (4)

A

s. pyogenes
s. agalactiae
s. dysagalactiae, s.equi

20
Q

SXT means

A

sulfamethoxazole trimethoprim

21
Q

diseases caused by s.pnuemoniae

A

meningitis
lobar pneumonia
otitis media

22
Q

staph
cause of UTI in women?
urine culture?

A

s. saprophyticus

10,000 CFU/mL

23
Q

lab test results for viridans strep

A

bile insoluble

optochin resistant

24
Q

test for identification of agalactiae?
rgt?
(+) result?

A

CAMP test
beta-lysin disk
bowtie appearance

25
Q

CAMP means

A

christie
atkinson
munch
petersen

26
Q

rgt for hippurate hydrolysis test?

(+) result?

A

Na hippurate + ninhydrin

purple

27
Q

rgt for catalase test

A

3% h2o2

28
Q

most virulent staph

A

s.aureus

28
Q

presumptive test for s.pneumoniae?
rgt?
result?

A

optochin susceptibility/p disk test
ethyhydroxycuprein hcl
>= 14 mm

29
Q

enterococcus (3)

A

e. faecalis
e. faecium
e. durans

32
Q

(+) result for neufeld quellung

A

swelling of capsule

33
Q

tests which differentiate grp D and grp E enterococci?

tests which E is (-)

A

bile esculin, 6.5% NaCl, PYR

nacl, pyr

34
Q

gro C,F,G is ____ to bacitracin

A

resistant

35
Q

grp which is only susceptible to SXT

A

C

36
Q

rgt used for modified oxidase test?

(+) result?

A

1% tetramethyl-p-phenylenediamene dihydrochloride

purple

36
Q

test for identification of s.pyogenes?

result?

A

bacitracin

susceptible

37
Q

test which detects cell bound coagulase?

(+) result? within?

A

slide method

clot formation within 30 sec

37
Q

normal flora of URT, GIT, GUT

A

viridans strep

38
Q

confirmatory test for s.pneumoniae?
rgt?
incubate for how long? with tempt
(+) result?

A

bile solubility
Na desoxycholate
3 hrs at 35 C
clear

38
Q

non enterococcus

A

s. bovis

s. equinis

40
Q

more specific test than bacitracin for pyogenes?
rgt?
(+) result?

A

L-pyrrolidonyl-alpha-naphthylamide test
0.01% p-dimethyl-aminocinnamaldehyde
cherry red

41
Q

known as nutritionally variant strep?
appearance in culture?
growth factors?

A

abiotrophia and granulicatella
satellites
thiol compounds (cystein,pyrodoxal,vitb6)

42
Q

not classified under lancefield?

species?

A

viridans strep

s.mitis,mutans,sanguis

43
Q

give 5 charcteristics of staph

A 
catalase-producing bacteria
facultative anaerobe
non motile
non spore forming
glucose fermenters
43
Q

gamma hemolytic strep

A

e. faecalis

e. facium

Microbiology (6 decks)

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