Molecular pathology Flashcards
What are techniques of molecular pathology?
In situ hybridization Polymerase chain reaction Restriction fragment length polymorphism Northern blot Southern blot Western blot Flow cytometry
What is in situ hybridization?
study of cells content
What is PCR?
amplification of DNA/RNA
What is restriction fragmentation length polymorphism
gene deletions are insertion in disease
What is Northern blot?
gene exprssion
detects RNA
What is Southern blot?
detects DNA sequence
What is western blot?
antibodies to separated proteins
What is flow cytometry
cell surface markers
What is laboratory clinical medicine divided into?
Immunohematology (blood banking) Chemistry Hematology Urinalysis Coagulation Serology Microbiology Molecular cytogenetics
What does pathology encompass about a disease?
all aspects of a disease
What are all aspects of a disease?
Cause (etiology)
Mechanism of development (pathogenesis)
Alteration in cells and organs
Morphologic/pathologic changes
Functional consequences (clinical signs & symptom)What are the etiology (causes) of diseases?
Genetic (e.g., Mendelian inherited mutations)
Acquired (e.g., infectious, nutritional, chemical, physical).
Disease-associated gene variants (polymorphisms) & environment
