Biology Cells part 2 Flashcards
Protein variety
There are 20 different types of natural amino acids so many different types of proteins can be made
Protein Structure
A typical protein is made up of about 400 amino acid.
The 3D shape of a protein determines what it can interact with which determines its purpose
Fibrous proteins
Make up structures such as bone and connective tissue, can take the form of rope like fibres
Keratin: makes up hair and nails
Globular-structural proteins
Structural component of cell membrane involved in transport in and out of cell
Protein channel and protein carrier
Globular-Enzymes
Catalyse biological reactions
Catalase, pepsin, phosphorylase
Globular-hormones
Communication between different parts of the body
Insulin:released from pancreas
Globular-antibodies
Part of the immune system, produced by white blood cells to destroy viruses and bacteria
Catalyst
Substance which speeds up a chemical reaction, is unchanged by reaction, made of protein, lower the activation energy
Enzyme
Biological catalyst which speeds up biochemical reactions and is unchanged after reaction, made of protein, made by all living cells
Enzyme specificity
Each enzyme is specific to it’s own substrate so each enzyme only catalyses one reaction, substrate enzyme products
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Example of degradation enzyme
Protein Pepsin Polypeptide
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Example of synthesis enzyme
Glucose-1-phosphate phosphorylase Starch
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Degradation enzymes
Break down the substrate molecules into smaller products
Induced fit
An enzymes active site fits the shape of its substrate. When the substrate enters the active site the enzyme’s shape changes slightly to tighten round the substrate increasing the chance of a chemical reaction
Synthesis enzymes
Build up the substrate molecules to form larger products
Temperature and enzymes
An enzyme works best at its optimum temperature, 37 degrees Celsius, at low temperatures enzymes become inactive but at high temperatures they are denatured
pH and enzymes
Enzymes have an optimum pH but also have a working range around that pH. If the pH is below or above the working range the enzyme will become denatured
Genetic engineering
Pieces of a chromosome are transferred from one type of organism into a bacterium or another organism
Bacterial cells
Contain a single chromosome and rings of genes called plasmids, bacterial cells can be used in genteel engineering to create insulin and human growth hormone
Genetic engineering steps
- useful gene identified
- Gene removed from chromosomes using enzymes
- DNA (plasmid) taken from bacterium
- Gene placed into the plasmid of host bacterium
- bacteria multiply with new gene