4.7.2013(micro-gram Positive Bacteria) Flashcards

0
Q

Temperature of culture of staph for pigment production

A

22 C

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1
Q

Selective medium for staphylococci

A

Salt milk agar(8-10% Nacl)
Mannitol salt agar(7.5% Nacl)
Ludlam Lithium chloride and tellurite media

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2
Q

Cause for golden yellow pigmentation of s.aureus colonies

A

Carotene

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3
Q

Gram positive bacteria that produces endotoxins

A

Listeria

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4
Q

Gram negative cocci

A

Neisseria

Veilonella

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5
Q

Gram positive bacteria with pili

A

Corynebacterium renale

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6
Q

Catalase positive gram positive cocci

A

S.aureus

Micrococci

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7
Q

Diff btw staph and Micrococci

A

Micrococci are
Oxidase Positive
Furazolidone resistant
Bacitracin sensitive

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8
Q

Staph which is slide coagulase positive but tube coagulase negative

A

S.lugdunensis

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9
Q

Slide coagulase positivity is because of

A

Bound coagulase(clumping factor)

Tube coagulase test requires free coagulase which needs coagulase reacting factor in plasma

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10
Q

Which is a virulence factor free or bound coagulase?

A

Free coagulase(it is heat Labile)

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11
Q

Basis of coagglutination reaction

A

Protein A of staph.aureus which binds to Fc fragment of IgG

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12
Q

Most consistent association of virulence of S.aureus

A

Coagulase

Fermentation of mannitol

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13
Q

Factor of S.aureus that exhibits hot cold phenomenon

A

Beta hemolysin

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14
Q

Coagulase acts by

A

Activation of prothrombin

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15
Q

Toxin associated with community acquired MRSA

A

Leukocidin/panton valentine toxin

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16
Q

Hemolysins in S.aureus

A

A,B,G,D

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17
Q

Vomiting in staph food poisoning

A

Activation of vagus and vomiting centre

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18
Q

The S.aureus toxin that is inactivated at 70C and activated paradoxically at 100 c

A

Alpha Hemolysins

At 100 c an inhibitor is inactivated

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19
Q

Bio component toxins of s.aureus

A

Leukocidin

Gamma hemolysin

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20
Q

Hemolysins of staphylococci that lyses only sheep RBC

A

Beta hemolysin

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21
Q

Exfoliative toxin of staph belongs to

A

Bacteriophage II group

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22
Q

Epidermolytic toxin causes

A
Ritter disease(children)
TEN(adults)
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23
Q

Types of Exfoliative toxin of S.aureus

A

A - heat stable,chromosome mediated

B- heat Labile,plasmid mediated

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24
Q

Most common type of enterotoxin

A

Type A

25
Q

Staph enterotoxin is heat _______

A

Stable

26
Q

Toxic shock syndrome toxin belongs to

A

Phage group 1

27
Q

Rx of toxic shock syndrome

A

Clindamycin

28
Q

TSST

A

Enterotoxin F
Pyrogenic exotoxin C(most common)
Enterotoxin B,C

29
Q

Examples for super antigens

A

Staph- TSST,Exfoliative toxin,enterotoxins
Strep- strep Pyrogenic exotoxin A,B,C
mycoplasma arthritidis,yersinia enterocolitica
Malassezia furfur
Virus-EBV,CMV,HIV,rabies

30
Q

Mechanism of MRSA

A

Chromosomally mediated mecA gene

Altered pencillin binding protein 2a

31
Q

BORSA

A

Borderline oxacillin resistant s.aureus

Hyper production of betalactamase

32
Q

Rx of MRSA

A

Vancomycin(DOC)
Linezolid
Teicoplanin
Streptogramin

33
Q

Newer drugs for MRSA

A

Daptomycin
Ceftabiprole
Tigecycline
Oritavancin

34
Q

VISA

A

Vancomycin intermediate s.aureus- MIC 8-16ug/ml

VRSA-MIC 32ug/ml

35
Q

Mechanism of VRSA

A

Increased thickness of cell wall

36
Q

Rx of VRSA

A

All drugs given for MRSA except vanco and teicoplanin

37
Q

S.aureus is MC agent for

A
Skin and soft tissue infection
Postoperative parotits
Postoperative wound infection
Botryomycosis
Paronychia
Epidural abscess
Tropical pyomyositis
Osteomyelitis
38
Q

Most common cause of acute bacterial Myositis

A

Group A streptococci

39
Q

Most common cause of prosthetic valve endocarditis

A

Saph.epidermidis

40
Q

MC cause of subacute endocarditis

A

Strep viridans

41
Q

Most common cause of late prosthetic valve endocarditis (>12months)

A

Strep viridans

42
Q

Most common method for typing of s.aureus

A

Phage typing

43
Q

Mechanism of penicillin resistance in S.aureus

A

Beta lactamase transferred by transduction

44
Q

Most common site of colonisation of staph aureus

A

Anterior nares

45
Q

Most common way of spread of s.aureus in hospital

A

Hands of hospital staff

46
Q

Most effective way to prevent hospital infection due to s.aureus

A

Hand washing

47
Q

MC CONS

A

S.epidermidis

48
Q

S.saprophyticus causes

A

UTI in sexually active females

49
Q

Diff btw s.epidermidis and saprophyticus

A

Saprophyticus is novobiocin resistant

50
Q

Optimum temperature for s.aureus
Pigment production
Enterotoxin production
MRSA expression

A

Pigment- 22 C
Enterotoxin- 37 C (same as opt temp for S.aureus growth)
MRSA-30 C in 4% Nacl

51
Q

Foods associated with staph food poisoning

A

Meat
Fish
Milk
Milk products

52
Q

Transfer of drug resistance in staph is by

A

Transduction(common)

Conjugation

53
Q

Sites of colonisation of staph

A
Skin of
 Anterior nares
 Perineum
 Axilla
 Groin
54
Q

Role of PBP

A

Trans or carboxy peptidase essential for cell wall synthesis

55
Q

Theromonuclease test for staph

A

Only aureus is positive

56
Q

Phosphatase test for staph

A

Aureus and epidermidis are positive

57
Q

Causes of botryomycosis

A
S.aureus
CONS
Streptococcus
Peptostreptococcus
Pseudomonas
E.coli
Proteus
58
Q

MC route of staph infection

A

Skin

59
Q

Lancefield grouping is based on

A

Carbohydrate antigen on cell wall of beta Hemolytic streptococci

20 subtypes,A to V except I and J

60
Q

Lancefield antigens are extracted by

A

HCl
Formamide(fuller)
Enzymatic(maxted)
Autoclaving

61
Q

Griffith typing is based on

A

Proteins M,T,R

more than 80 M types are present