Nucleotides: The Building Blocks of Nucleic Acids Flashcards
1
Q
primary structure
A
- sequence of amino acids in a protein
2
Q
secondary structure
A
- first order folding
3
Q
tertiary structure
A
- second-order folding
- 3-D structure
4
Q
quaternary structure
A
- subunit interaction
5
Q
Nucleoside
A
Base + Sugar
6
Q
Nucleotide
A
Base + Sugar + Phosphate
7
Q
Sugar
A
- Ribose (RNA)
- Deoxyribose (DNA) - no OH in 2’ position
8
Q
Purines
A
- Adenine
- Guanine
- two membered rings
9
Q
Pyrimidines
A
- Cytosine
- Thymine - only DNA
- Uracil - only RNA
- single ring
10
Q
prime (‘)
A
- prevent numbering confusion with the atoms in the bases.
11
Q
bond between base and sugar
A
- N-glycosidic bond (N-9)
- covalently bonded to N
12
Q
P32
A
- common radioactive label
13
Q
Following the addition of nucleotides into DNA or RNA (nucleic acid)
A
- put P32 on the 5’ alpha position on the free nucleotides
14
Q
Following the transfer of a phosphate
A
- put P32 on the 5’ gamma position on the ATP
15
Q
Where are the phosphates?
A
May be carried in the 5’ or 3’ position
