5. Luminescence Flashcards
What is Photometry?
Photometry is the science of the measurement of light, in terms of its perceived brightness to the human eye.
What is Spectrometry?
Spectroscopy is the theoretical science, and spectrometry is the practical measurement i.e. application of that science.
What is spectrophotometry?
Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution.
What is absorption?
In physics, absorption of electromagnetic radiation is how matter takes up a photon’s energy — and so transforms electromagnetic energy into internal energy of the absorber (for example, thermal energy).
What is a chromophore?
A chromophore is the part of a molecule responsible for its color. The chromophore is a region in the molecule where the energy difference between two separate molecular orbitals falls within the range of the visible spectrum.
What is a fluorophore?
A fluorophore is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds.
What is fluorescence?
Fluorescence is photoluminescence as a result of singlet–singlet electronic relaxation (typical lifetime: nanoseconds).
What is luminescence?
Luminescence is spontaneous emission of light by a substance.
What is emission?
In physics, emission is the process by which a higher energy quantum mechanical state of a particle becomes converted to a lower one through the emission of a photon, resulting in the production of light.
What is phosphorescence?
Phosphorescence is photoluminescence as a result of triplet–singlet electronic relaxation (typical lifetime: milliseconds to hours).
What is chemiluminescence?
Chemiluminescence is the emission of light as a result of a chemical reaction.
What is bioluminescence?
Bioluminescence is a result of biochemical reactions in a living organism.
What is derivatisation?
Derivatisation is a technique used in chemistry which converts a chemical compound into a product (the reaction’s derivate) of similar chemical structure, called a derivative.
What is quenching?
Quenching refers to any process which decreases the fluorescence intensity of a given substance.
A variety of processes can result in quenching, such as excited state reactions, energy transfer, complex-formation and collisional quenching.
