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MBIO 360 > Module 12 > Flashcards

Module 12 Flashcards

1
Q

How does Proteus grow on media?

A

BAP- grey moist, swarming

MAC- NLF, may spread

XLD, HE, SS- good growth

Not inhibited in selective enrichment media

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2
Q

What is the ID of Proteus?

A

Glucose pos with gas

Lactose neg (NLF)

Sucrose neg for mirabilis and pos for vulgaris

H2S pos

K/A, gas, H2S for mirabilis
A/A, gas, H2S for vulgaris

Deaminase pos

Urease pos

Indole neg for mirabilis and pos for vulgaris

Ornithine pos for mirabilis and neg for vulgaris

Motile

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3
Q

What is the rapid ID for Proteus?

A

Swarming on BAP

Spot indole pos for vulgaris, neg for mirabilis

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4
Q

What plates are used to inhibit Proteus and prevent the swarming that may overwhelm other bacteria?

A

CNA/PEA

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5
Q

What is the clinical significance of Proteus?

A

NF

Nosocomial infections

Mirabilis is susceptible to ampicillin (vulgaris is resistant)

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6
Q

What is the treatment for Proteus?

A

Ciprofloxacin, third gen cephalosporins, meropenem, urinary drugs, piperacillin/tazonactam

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7
Q

What are the most commonly isolated Providencia species?

A

Rettgeri and stuartii

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8
Q

How does Providencia grow on media?

A

BAP- grey moist

MAC- NLF

XLD, HE, SS- NLF, no black centres

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9
Q

What is the ID results for Providencia?

A

Glucose pos with gas

Lactose neg (NLF)

Sucrose pos

H2S neg

K/A, may show has

ONPG neg

Deaminase pos

Urease variable

Ornithine neg

Indole pos

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10
Q

What is the clinical significance of Providencia?

A

Insignificant in stool cultures

Nosocomial infections

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11
Q

What is the treatment for Providencia?

A

Third or fourth gen cephalosporins, aztreonam, imipenem, some fluoroquinolones, aminoglycosides and SXT

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12
Q

How does Morganella morganii grow on media?

A

BAP- grey moist

MAC- NLF

XLD, HE, SS- NLF, no black centres

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13
Q

What are the ID results for Morganella?

A

Glucose pos with little gas

K/A

Lactose neg

Sucrose neg

H2S neg

Deaminase pos

Urease pos

ONPG neg

Indole pos

Ornithine pos

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14
Q

How is Morganella differentiated from Shigella?

A

Pos deaminase and urease

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15
Q

What is the clinical significance of Morganella?

A

NF

Nosocomial infections

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16
Q

How is Morganella treated?

A

Piperacillin, ticarcillin, third and fourth gen cephalosporins, carbapenems, aztreonam, fluoroquinolones, aminoglycosides and chloramphenicol

17
Q

What are the three species of Yersinia that are human pathogens?

A

Pestis

Psuedotuberculosis

Enterocolotica

18
Q

What is the optimum temp for Yersinia?

A

25-30°C

19
Q

How does Yersinia grow on media?

A

BAP- tiny grey moist overnight, 2-3mm after 36-48 hrs

MAC- tiny NLF

XLD, HE, SS- tiny NLF

20
Q

What media is used to isolate Yersinia?

A

CIN/YERS

21
Q

What are the ingredients of CIN/YERS?

A

Peptone and yeast

Mannitol and neutral red

Crystal violet

Cefsulodin

Irgasan

Novobiocin

22
Q

How do Yersinia colonies appear on CIN/YERS?

A

Dark red centre

Bullseye

23
Q

What is the clinical significance of Yersinia enterocolitica?

A

Not NF

Gastroenteritis

Septicaemia

24
Q

What is the treatment for Yersinia?

A

Aminoglycosides, SXT, 3rd gen cephalosporins, tetracyclines, fluoroquinolones, ciprofloxacin, levofloxacin, nalidixic acid

25
Q

What species of Proteus are most commonly isolated?

A

Mirabilis and vulgaris

MBIO 360 (14 decks)

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