Module 13

Question 1

What ID tests are used for NFB?

Answer 1

API 20E/ API NFT

Vitek

Gram

MAC

OF- +/-

Oxidase

Motility

TSI- K/K

Question 2

How can gram positive rods that are over decolourized be distinguished from NFB?

Answer 2

KOH string test- gram neg show string

Vancomycin disc- gram neg resistant

Question 3

How can gram neg cocci that are elongated differentiated from NFB?

Answer 3

Gram from liquid medium- more prominent bacillary forms

Smear from edge of zone of inhibition shows bacillary forms

Question 4

What are the components of OF media?

Answer 4

Low peptone concentration

Bromthymol blue

1% concentration of carbohydrates

Question 5

What method is used to perform motility testing?

Answer 5

Wet prep

Question 6

What is MH agar used for?

Answer 6

Demonstrates pigment production

Oxidase testing

Antimicrobial sensitivities

Question 7

What are the characteristics of nonfermentative gram negative bacilli (NFB)?

Answer 7

All fail to ferment carbs

Acid not produced in carb fermentation media without O2, anaerobic tube of OF test or TSI butt

Widely distributed

Low grade pathogens