5.3 Classification of Indicator Labeled Immunoassays

Question 1

Principle of this is labeled and unlabeled analytes can compete for the same site on the antibody

a. Competitive
b. Noncompetitive

Answer 1

a

Question 2

In competitive immunoassay, the measured signal is inversely correlated to the concentrations of analyte in the sample

T or F

Answer 2

T

Question 3

In competitive, the higher the signal, the lower the antigen concentration

T or F

Answer 3

T

Question 4

NOTE: Label produces the signal in competitive

Question 5

For Competitive, this includes Radioimmunoassay

T or F

Answer 5

T

Question 6

A highly specific and sensitive assay that involves the use of radioisotopes as it label or marker

Answer 6

Radioimmunoassay

Question 7

Radioimmunoassay, What is the means to measure the analyte?

Answer 7

Scintillation counter (Gamma COunter)

Question 8

This employs enzymes as the label for competitive immunoassay

Answer 8

EMIT (Enzyme multiplied immunoassay technique)

Question 9

EMIT usually used Heterogeneous format

T or F

Answer 9

F

Homogeneous format

Question 10

The application of EMIT is the detection of small molecules such as Hormone, especially _____

Answer 10

Drugs (Board exam)

Question 11

Application for this is to detect small molecules such as Drugs

Answer 11

EMIT

Question 12

Speciment for drug testing using EMIT?

Answer 12

Urine

Question 13

Advantage of EMIT?

Answer 13

No washing

Question 14

The higher signal for EMIT, The lower amount for drugs

T or F

Answer 14

T

Question 15

A non classical 2 stage competitive immunoassay that can be used to detect illegal and therapeutic drugs

Answer 15

FPIA (Fluorescence polarization immunoassay

Question 16

Specimen for FPIA when it comes to drugs

Answer 16

Urine

Question 17

In FPIA:

(+) = Slower / decreased polarization

(-) = Faster / Increased polarization

T or F

Answer 17

T

Question 18

Detection of either Ag or Ab in biological sample

a. Competitive
b. Non competitive

Answer 18

b

Question 19

Ag and Ab are permitted to interact without deliberate introduction of competing molecules

Answer 19

Non compettive

Question 20

Most commonly used immunoassay in noncompetitive?

Answer 20

ELISA

Question 21

Non-competitive:

Method uses a labeled primary antibody that reacts directly with the Ag

a. Direct detection
b. Indirect detection

Answer 21

a

Question 22

Direct Elisa is a heterogeneous assay

T or F

Answer 22

T

Question 23

Direct elisa contains washing

T or F

Answer 23

T

Question 24

In Direct Elisa, If the antigen is bound to the assay, is the primary antibody labeled?

Answer 24

yes

Question 25

Application for Direct Detection?

Answer 25

Immunohistochemistry

Question 26

Non-competitive: Method is the most popular for ELISA a. Direct detection b. Indirect detection

Answer 26

b

Question 27

Non-competitive: More sensitive a. Direct detection b. Indirect detection

Answer 27

b

Question 28

In indirect detection, if the antigen is bound to the assay, the primary antibody is unlabeled or labeled?

Answer 28

Unlabeled

Question 29

In indirect detection, after the primary antibody is bound to the bound antigen, the secondary antibody is added which is labeled or unlabeled?

Answer 29

Labeled

Question 30

In indirect detection, The secondary antibody is an anti _____ that binds to the ___ portion of the antibody

Answer 30

IgG Fc

Question 31

Non-competitive: Has strong signal that even the smallest amount can be detected a. Direct detection b. Indirect detection

Answer 31

b

Question 32

This assay is sensitive, Robust that is in ELISA assay format where analyte (To be measured bound between 2 antibodies which are capture Ab;Detector Ab

Answer 32

Sandwich assay

Question 33

Ab source for Sandwich assay?

Answer 33

Rabbit and Mice