5.5) Antibodies Flashcards
(10 cards)
What is the role of the disulfide bridge in forming the quaternary structure
of an antibody?
It join two polypeptides
What are antibodies?
They are proteins synthesised by B cells
What is formed when antibodies attach to antigens
They form an antigen-antibody complex
What is the structure of antibodies?
They are made up of forming 4 different polypeptide chains
What is the binding site on antibodies known as?
The variable region
What is the remaining of the antibody called?
A constant region
What is a monoclonal antibody ?
It’s antibodies that are isolated produced by a single clone of cells
–> Antibodies with the same tertiary structure
What are some of the uses of monoclonal antibodies?
- Cancer Treatment
- Transplant surgery
*Immunoassay ( method of calculating the amount of substance in a mixture)
What is the role of the antibodies in the ELISA test
- Antibody attaches to antigen (complimentary to antigen in shape)
- Antibody with enzyme attached is added;
- Antibody attaches to antigen;
- The second antibody attaches to first antibody
- Substrate/solution added and colour changes
Describe and explain the role of antibodies in stimulating phagocytosis.
- Antibodies bind to antigen
- Antibodies cause clumping
