EB22 Flashcards
How are mosquitos collected
human landing catches/spray
landing catches; ethically dubious: catch them on skin at feeding time
Spray collections: spray houses in the morning and put sheet outs to catch insects
how is mosqutio DNA collected
max 1ug per mosquito could be limiting for some applications.
*rapid tech means able to sequence genome of single mosquito
what markers can be used for genome comparisons
- allozymes and chromosomal banding patterns used ot be uysed.
- mtDNA easy to amplify (many copies)
- microsatellites
- AFLPs: amplified fragment length polymorphisms
- Nuclear gene sequences
- SNPs
what are the pros and cons of using mtDNA
Pro: easy to amplify, easy to get sequence data
con: essentail 1 locus so selection could obscure demography
what are the pros and cons of using microsatellites:
pros: many developed in anopheles, easy to amplify, multi locus
cons: limited no. can be processed, no good model of evolution.
what are the pros and cons of nuclear gene sequences
pros: multilocus, sequence data
cons: practically limited to a small no. and must be careful of selection in coding regions.
What are the various methods of stat analysis of population diversity and structure
- Nt diversity: effective population size
- Neutrality tests: selection or demographic changes
- HWE: assortative mating or selection
- Fst:differentiation between populations (divergence)
What are examples of more complex analysis of population strucutre and population hisotry
- Haplotype networks: similar to phylogeny reconstruction
- Clustering analysis: cluster data to try and identify whether two popualtions/three/four/five are linked and then find cluster to best fit the data
- Linkage: `
what is the challenge in population history
how can this be done
to tease apart ongoing gene flow from popualtion history
done by modelling genetic data under different demographic scenarios (Lamarc, SPATCH, dadi, ms ABC.
What is an example of a rapidly spread resistance gene
kdr
*single pathotype shared across Africa and between species
how we infer the source (single or multiple origins) or a mutation
looking at the flanking DNA around the mutation can help infer the source, if it spread from one source than that flanking DNA would spread with it.
What was found to be the origin of Kdr
why is this info useful
multiple origins of this mutation
followed by wide georgraphical spread, giving info about gene flow
this info and that of the time series data can tell us how our transgene might spread through content wide mosqutio populations
What are the 4 types of spread of a transgene that need to be taken into account
- spatial spread: physical barriers to gene flow
- temporal spread: across dry seasons?
- spread between closely related species
- resistant genotype
What could the potential pitfalls be of the spatial spread of a transgene
potential stopped by areas with no humans
genetic data showed no barriers between large parts of Africa, spatial spread is possible. (via numerous mtDNA and microsat studies)
** rift valley could be a potential barrier
What could the potentially pitfalls of temporal spread of a transgene
experiment: same population measured before and after dry season
found they woukld not be at risk in dry season