Intracellular Membrane Transport Flashcards
The major organelles involved in intracellular tranpsort
The ER and the Golgi Apartus
The function of the ER
Protein production in ribosomes
The main coat proteins we need to know
- Clathrin
- COPI
- COPII
The function of clathrin
Binds to coat plasma membrane and between endosomes and the Golgi; Links actin and cytoskeleton
The function of COPI
Secretory pathway; bud from the Golgi
The function of COPII
Secretory pathway; bud from the ER
Adaptor proteins (Sec, AP2)
Alter conformations when it binds to a certain phosphoinositide which exposes it for cargo receptors
GTPases (Sar)
Control the assembly of COPI and COPII coats on Golgi and ER and clathrin on endosomes. Sar specifically controls assembly of COPII at the ER membrane
Phosphoinositides
Marks organelles and membrane domains with (de)phosphorylation events
Rab/Rab effectors
Guide transport vesicles to their target membranes and direct the vesicles
SNARE
Regulators that mediate the fusion of lipid bilayers
GAP/GEF
GAP’s are GTPase activating factors that inactivate proteins by triggering the hydrolysis of GTP. GEF’s activate by catalyzing the exchange of GDP for GTP.
Dynamin
Pinches off clathrin coated vesicles with a binding domain powered by GTP hydrolysis